Non-immunoglobulin-associated DNA rearrangements in mouse plasmacytomas.
Non-immunoglobulin-associated DNA rearrangements in mouse plasmacytomas.
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小鼠浆细胞瘤中非免疫球蛋白相关的 DNA 重排。
DOI：
10.1073/pnas.79.13.4175
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发表时间：
1982
影响因子：
11.1
通讯作者：
Marcu,KB
中科院分区：
文献类型：
--
作者：
Harris,LJ;Lang,RB;Marcu,KB
我们描述了浆细胞瘤中一类DNA重排的特征。这些重组事件涉及一个DNA序列，其起源在重链恒定区基因CH的基因座之外。因此，我们选择将该序列称为非免疫球蛋白相关重排DNA（NIARD）。我们已经分离出两个流产重排的C α基因，产生的NIARD事件从α生产的J558骨髓瘤。这些序列的限制性内切酶图谱揭示了NIARD中两个可能的重组位点，它们被大约6.5个DNA内切酶对（定义为5 '和3'位点）分开。NIARD重排发生在20个浆细胞瘤中的15个，包括γ 3-，γ 1-，γ 2b-，γ 2a-和α-生产者，但这种事件通常不涉及CH开关（S）区域。事实上，只有S alpha似乎接受NIARD。然而，NIARD没有经历重排在8个IgA生产杂交瘤测试。NIARD（22-内切酶对EcoRI片段）的一个生殖系拷贝在所有浆细胞瘤中保留。NIARD似乎不具有与S μ或S α同源的重复DNA序列。我们讨论了NIARD样序列重排在浆细胞瘤等位基因排斥和染色体易位事件中可能的作用和意义。
We have characterized a class of DNA rearrangements in plasmacytomas. These recombination events involve a DNA sequence whose origin is outside of the locus of the heavy chain constant region genes, CH. Therefore, we choose to refer to this sequence as non-immunoglobulin-associated rearranging DNA (NIARD). We have isolated two abortively rearranged C alpha genes, generated by NIARD events from the alpha-producing J558 myeloma. Restriction endonuclease maps of these sequences reveal two possible recombination sites in NIARD that are separated by approximately 6.5 kilobase pairs of DNA (defined as 5' and 3' sites). A NIARD rearrangement occurs in 15 out of 20 plasmacytomas tested, including gamma 3-, gamma 1-, gamma 2b-, gamma 2a-, and alpha-producers, but this event usually does not involve a CH switch (S) region. In fact, only S alpha appears to accept NIARD. However, NIARD did not undergo a rearrangement in eight IgA-producing hybridomas tested. One germ-line copy of NIARD (a 22-kilobase pair EcoRI fragment) is retained in all plasmacytomas. NIARD does not appear to possess repetitive DNA sequences homologous to S mu or S alpha. We discuss the possible role and implications of NIARD-like sequence rearrangements in allelic exclusion and chromosomal translocation events in plasmacytomas.