A microfluidic device for practical label-free CD4+T cell counting of HIV-infected subjects
A microfluidic device for practical label-free CD4+T cell counting of HIV-infected subjects
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DOI：
10.1039/b612966h
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发表时间：
2007-01-01
期刊：
影响因子：
6.1
通讯作者：
Toner, Mehmet
中科院分区：
文献类型：
--
作者：
Cheng, Xuanhong;Irimia, Daniel;Toner, Mehmet
在资源有限的情况下，迫切需要实用的艾滋病毒诊断方法。虽然HIV感染可以使用简单、快速、侧流免疫测定法进行诊断，但HIV疾病分期和治疗监测需要准确计数特定的白色血细胞亚群，即CD 4 + T淋巴细胞。为了解决目前昂贵的，技术要求高和/或耗时的方法的局限性，我们已经开发了一个简单的CD 4计数微流控装置。该设备使用在差示剪切流下操作的细胞亲和色谱，直接从10微升未处理的未标记全血中高效特异性分离CD 4 + T淋巴细胞。在光学显微镜下以快速、简单和无标记的方式获得CD 4计数。在我们的设备中测定的CD 4计数与HIV阳性受试者中通过常规流式细胞术测定的CD 4计数在广泛的绝对CD 4计数范围内匹配（R-2 = 0.93）。这种CD 4计数微型设备可用于在护理点和资源有限的环境中进行简单，快速和负担得起的CD 4计数。
Practical HIV diagnostics are urgently needed in resource-limited settings. While HIV infection can be diagnosed using simple, rapid, lateral flow immunoassays, HIV disease staging and treatment monitoring require accurate counting of a particular white blood cell subset, the CD4+ T lymphocyte. To address the limitations of current expensive, technically demanding and/or time-consuming approaches, we have developed a simple CD4 counting microfluidic device. This device uses cell affinity chromatography operated under differential shear flow to specifically isolate CD4+ T lymphocytes with high efficiency directly from 10 microlitres of unprocessed, unlabeled whole blood. CD4 counts are obtained under an optical microscope in a rapid, simple and label-free fashion. CD4 counts determined in our device matched measurements by conventional flow cytometry among HIV-positive subjects over a wide range of absolute CD4 counts (R-2 = 0.93). This CD4 counting microdevice can be used for simple, rapid and affordable CD4 counting in point-of-care and resource-limited settings.