Consequences of Hydrocarbon Contamination for Wettability and Protein Adsorption on Graphite Surfaces
Consequences of Hydrocarbon Contamination for Wettability and Protein Adsorption on Graphite Surfaces
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DOI：
10.1021/acs.jpcc.5b02948
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发表时间：
2015-06-04
影响因子：
3.7
通讯作者：
Urbassek, Herbert M.
中科院分区：
文献类型：
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作者：
Muecksch, Christian;Roesch, Christina;Urbassek, Herbert M.
我们目前的结果，从分子动力学模拟和接触角测量石墨显示，这种表面确实是本质上温和的亲水性，与普遍的信念。碳氢化合物污染，已知是通常观察到的石墨疏水性的来源，也影响蛋白质吸附过程，如本研究所示。在这项工作的计算部分，乙烷被用作模型烃，通过减少水表面和蛋白质表面相互作用的表面上的行为。这种污染导致更高的水接触角。蛋白质吸附的过程进行了研究，胰岛素的例子揭示了吸附强度的降低，尽管表面被污染时更疏水。虽然蛋白质在污染表面上没有变性，但进一步的过程，如蛋白质对碳氢化合物的置换，可能会在更长的时间尺度上发生。总之，我们认为，蛋白质吸附速度比在污染的表面上纯的分子动力学时间尺度的ns。
We present results from molecular dynamics simulations and contact angle measurements on graphite showing that this surface is indeed intrinsically mildly hydrophilic contrary to the common belief. Hydrocarbon contamination, known to be the source for the usually observed hydrophobic property of graphite, also affects protein adsorption processes as shown in this study. In the computational part of this work ethane was used as a model hydrocarbon which acts on the surface by reducing the water-surface and protein-surface interactions. This contamination then results in higher water contact angles. The process of protein adsorption is studied for the example of insulin revealing a reduction in adsorption strength despite the surface being more hydrophobic when contaminated. Although the proteins did not denature on the contaminated surfaces, further processes, such as the displacement of hydrocarbons by the protein, may occur on a longer time scale. In conclusion, we argue that proteins adsorb faster on pure than on contaminated surfaces with respect to the molecular dynamics time scale of ns.