Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature.
Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature.
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DOI：
10.1371/journal.pone.0245582
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Drury S
中科院分区：
文献类型：
--
作者：
Lindrose AR;McLester-Davis LWY;Tristano RI;Kataria L;Gadalla SM;Eisenberg DTA;Verhulst S;Drury S
近年来，端粒长度（TL）作为各种环境暴露和疾病的生物标志物的使用有所增加。已经开发了各种方法来测量端粒长度。基于聚合酶链反应（PCR）的方法由于其高通量的能力，仍然广泛用于基于人群的研究。虽然多项研究评估了不同TL测量方法的重复性和再现性，但结果各不相同。我们对TL测量交叉方法比较研究进行了文献综述，其中包括2002年1月1日至2020年5月25日期间发表的基于PCR的方法。共发现25篇文章符合纳入标准。论文进行了审查，质量的方法学报告的样本和DNA质量，PCR检测的特点，样本盲法，分析方法，以确定最终的TL。总体而言，根据基于qPCR的TL测量的两种不同报告指南的评估，方法学报告较低。报告的方法之间的相关性范围很广（通过Pearson's r评估），很少有研究使用推荐的类内相关系数（ICC）评估测定重复性和方法学比较。几乎所有研究的样本量都小于100，这引起了对统计功效的担忧。总体而言，这篇评论发现，目前的文献之间的关系TL测量方法缺乏有效性和科学严谨性。鉴于这些发现，我们提出了基于PCR的TL测量方法的报告指南，以及分析测定重复性（ICC）对横截面和纵向研究统计能力的影响的结果。具有严格方法学和统计学报告、足够样本量和盲法的额外跨实验室研究对于准确确定测定重复性和可复制性以及TL测量方法之间的关系至关重要。
Use of telomere length (TL) as a biomarker for various environmental exposures and diseases has increased in recent years. Various methods have been developed to measure telomere length. Polymerase chain reaction (PCR)-based methods remain wide-spread for population-based studies due to the high-throughput capability. While several studies have evaluated the repeatability and reproducibility of different TL measurement methods, the results have been variable. We conducted a literature review of TL measurement cross-method comparison studies that included a PCR-based method published between January 1, 2002 and May 25, 2020. A total of 25 articles were found that matched the inclusion criteria. Papers were reviewed for quality of methodologic reporting of sample and DNA quality, PCR assay characteristics, sample blinding, and analytic approaches to determine final TL. Overall, methodologic reporting was low as assessed by two different reporting guidelines for qPCR-based TL measurement. There was a wide range in the reported correlation between methods (as assessed by Pearson’s r) and few studies utilized the recommended intra-class correlation coefficient (ICC) for assessment of assay repeatability and methodologic comparisons. The sample size for nearly all studies was less than 100, raising concerns about statistical power. Overall, this review found that the current literature on the relation between TL measurement methods is lacking in validity and scientific rigor. In light of these findings, we present reporting guidelines for PCR-based TL measurement methods and results of analyses of the effect of assay repeatability (ICC) on statistical power of cross-sectional and longitudinal studies. Additional cross-laboratory studies with rigorous methodologic and statistical reporting, adequate sample size, and blinding are essential to accurately determine assay repeatability and replicability as well as the relation between TL measurement methods.
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端粒：一种倍性 - 敏锐的方法，用于从整个基因组测序数据中估算端粒长度。
影响因子：
4.6
作者：
Farmery JHR;Smith ML;NIHR BioResource - Rare Diseases;Lynch AG
通讯作者：
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Cawthon RM
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通讯作者：
Aviv A
DOI：
10.1016/j.neo.2018.02.007
发表时间：
2018-05
期刊：
Neoplasia (New York, N.Y.)
影响因子：
--
作者：
Dahlgren PN;Bishop K;Dey S;Herbert BS;Tanaka H
通讯作者：
Tanaka H