AN INTEGRATED APPROACH TO STUDYING REGULATION OF PRODUCTION OF THE ANTIBIOTIC METHYLENOMYCIN BY STREPTOMYCES-COELICOLOR A3(2)
AN INTEGRATED APPROACH TO STUDYING REGULATION OF PRODUCTION OF THE ANTIBIOTIC METHYLENOMYCIN BY STREPTOMYCES-COELICOLOR A3(2)
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DOI：
10.1128/jb.174.5.1487-1494.1992
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发表时间：
1992-03-01
影响因子：
3.2
通讯作者：
OLIVER, SG
中科院分区：
文献类型：
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作者：
HOBBS, G;OBANYE, AIC;OLIVER, SG
对天蓝色链霉菌A3（2）亚甲基霉素生物合成的调控进行了生理和分子生物学研究。 建立了一种简便、可靠的检测方法。 研究了S.在确定的培养基中生长的腔棘鱼培养物被阐明：需要容易同化的碳和氮源。 在这些条件下，亚甲基霉素在生长期后期，即从指数生长过渡到线性生长时产生。 如果培养基中的磷酸盐浓度保持较高，则可以合成亚甲基霉素，但不能合成该菌株可以产生的其他次级代谢产物。 这些条件被用来研究从初级代谢到次级代谢的过渡过程中的亚甲基霉素基因簇的转录。 至少一个mmy转录单位的生物合成基因似乎在mmr抗性决定子之前转录。 并对亚甲基霉素诱导mmr转录的可能性进行了讨论。
A physiological and molecular biological study was made of the control of methylenomycin biosynthesis by Streptomyces coelicolor A3(2). A simple and reliable assay for this antibiotic was developed. Conditions that permit the synthesis of methylenomycin by S. coelicolor cultures grown in defined medium were elucidated: a readily assimilated carbon and nitrogen source is required. Under these conditions methylenomycin is produced late in the growth phase, at the time of transition from exponential to linear growth. Provided that the phosphate concentration in the medium is kept high, there is synthesis of methylenomycin but not of the other secondary metabolites that this strain can produce. These conditions were used to study the transcription of the methylenomycin gene cluster during the transition from primary to secondary metabolism. The biosynthetic genes of at least one of the mmy transcription units appear to be transcribed before the mmr resistance determinant. The possibility that methylenomycin induces the transcription of mmr is discussed.