West Nile Virus Monitoring of Migratory and Resident Birds in Germany
West Nile Virus Monitoring of Migratory and Resident Birds in Germany
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DOI：
10.1089/vbz.2009.0236
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发表时间：
2010-10-01
影响因子：
2.1
通讯作者：
Groschup, Martin H.
中科院分区：
文献类型：
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作者：
Seidowski, Diana;Ziegler, Ute;Groschup, Martin H.
西尼罗河病毒(West Nile Virus，WNV)是一种蚊媒黄病毒，在野生鸟类种群中自然传播。该病毒还能够感染广泛的脊椎动物物种。人和马是高度敏感的，可能会发展成轻微的流感样疾病，以及导致死亡的严重脑炎。最近，西尼罗河病毒被发现在德国附近的国家传播，如法国、捷克共和国、意大利、奥地利和匈牙利。鉴于这种流行病情况，不能排除它向德国蔓延的可能性。由于最近没有关于德国西尼罗河病毒情况的数据，我们进行了一项血清学调查，以揭示野鸟中的西尼罗河病毒抗体。对2005-2009年期间收集的72种候鸟和留鸟的2700多份血液样本进行了免疫荧光检测，部分检测了微型病毒中和试验。免疫荧光法在11种野生鸟类中检测到西尼罗河病毒反应性抗体。同样，在属于10种候鸟的候鸟中发现了西尼罗河病毒中和抗体，但在留鸟中没有发现。根据留鸟中没有西尼罗河病毒反应性抗体，以及所有调查的禽类样本中都没有西尼罗河病毒特异性RNA的情况，目前没有证据表明德国存在西尼罗河病毒传播。
West Nile virus (WNV) is a mosquito-borne flavivirus naturally circulating in wild bird populations. The virus is also capable to infect a broad range of vertebrate species. Humans and equines are highly susceptible and can develop mild flu-like illnesses as well as severe encephalitis leading to fatalities. Most recently, WNV was found to circulate in countries close to Germany, such as France, Czech Republic, Italy, Austria, and Hungary. Given this epidemiological situation its spread to Germany cannot be ruled out. As no data on the WNV situation were available for Germany for the most recent past, we have conducted a serological survey to reveal WNV antibodies in wild birds. More than 2700 blood samples from migratory and resident birds representing 72 species that were collected during 2005-2009 were tested using an immunofluorescence assay and partly by micro-virus neutralization test. By immunofluorescence assay WNV-reactive antibodies could be demonstrated in 11 wild bird species. Similarly, WNV-neutralizing antibodies were revealed in migratory birds belonging to 10 species, but not in resident birds. According to the absence of WNV-reactive antibodies in resident birds and the absence of WNV-specific RNA in all investigated bird samples, there is currently no evidence for a WNV circulation in Germany.