Optimization of naringenin and p-coumaric acid hydroxylation using the native E-coli hydroxylase complex, HpaBC
Optimization of naringenin and p-coumaric acid hydroxylation using the native E-coli hydroxylase complex, HpaBC
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DOI：
10.1002/btpr.2185
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发表时间：
2016-01-01
影响因子：
2.9
通讯作者：
Koffas, Mattheos A. G.
中科院分区：
文献类型：
--
作者：
Jones, J. Andrew;Collins, Shannon M.;Koffas, Mattheos A. G.
黄酮类化合物是一类具有生物活性的天然产物，在体内外均具有独特的生物活性。从植物来源中提取类黄酮受到其天然丰度低的限制，并且通常导致难以分离的产物混合物。然而，由于最近的进展，植物天然产物的微生物生产已发展为类黄酮生产的有前途的替代方案。通过对培养基、诱导温度、诱导点和底物延迟时间的优化，我们证明了迄今为止使用天然E.大肠杆菌羟化酶复合物。我们还显示了HpaBC对单羟基化黄烷-3-醇阿夫泽莱欣的体内活性的第一个证据，儿茶素产物滴度为34.7 +/- 1.5mg/L。这项工作证实了HpaBC在实现各种邻羟基化类黄酮和类黄酮衍生产物在E.杆菌(c)2015年美国化学工程师学会生物技术。程序，32：21-25，2016
Flavonoids are a growing class of bioactive natural products with distinct and interesting bioactivity both in vitro and in vivo. The extraction of flavonoids from plant sources is limited by their low natural abundance and commonly results in a mixture of products that are difficult to separate. However, due to recent advances, the microbial production of plant natural products has developed as a promising alternative for flavonoid production. Through optimization of media, induction temperature, induction point, and substrate delay time, we demonstrate the highest conversion of naringenin to eriodictyol (62.7 +/- 2.7mg/L) to date, using the native E. coli hydroxylase complex, HpaBC. We also show the first evidence of in vivo HpaBC activity towards the monohydroxylated flavan-3-ol afzelechin with catechin product titers of 34.7 +/- 1.5mg/L. This work confirms the wide applicability of HpaBC towards realizing efficient de novo production of various orthohydroxylated flavonoids and flavonoid derived products in E. coli. (c) 2015 American Institute of Chemical Engineers Biotechnol. Prog., 32:21-25, 2016