The Effect of Lidocaine on Cholinergic Neurotransmission in an Identified Reconstructed Synapse
The Effect of Lidocaine on Cholinergic Neurotransmission in an Identified Reconstructed Synapse
复制标题
利多卡因对已确定的重建突触中胆碱能神经传递的影响
作者：
S. Onizuka;T. Kasaba;M. Takasaki
背景技术背景：由于突触前神经元识别的困难和体内中枢神经系统的复杂性，利多卡因对胆碱能突触传递的突触前作用尚不清楚。为了阐明利多卡因对胆碱能突触的影响，我们重建了一个培养的体细胞化学突触模型，包括两个确定的内脏背4（VD 4）和左足e-1（LPeD 1）神经元从蜗牛，滞流，在体外，并使用它来确定利多卡因如何影响胆碱能突触传递。方法：在由VD 4和LPeD 1神经元组成的化学突触传递模型中记录对乙酰胆碱的反应和兴奋性突触后电位（EPSP）幅度。在突触前VD 4和突触后LPeD 1神经元中，在电压钳下进行乙酰胆碱电流测量。结果：利多卡因可抑制突触后神经元的EPSP和对乙酰胆碱的反应。突触前神经元的EPSP振幅以电压依赖性方式降低，利多卡因使EPSP振幅的电压依赖性失活曲线超极化移位。结论：利多卡因通过突触前神经元的膜电位去极化抑制胆碱能突触传递，并使EPSP振幅电压依赖性失活。
BACKGROUND: The presynaptic effect of lidocaine on cholinergic synaptic transmission is unclear because of the difficulty in identifying presynaptic neurons and the complexity of the central nervous system in vivo. To clarify the effect of lidocaine on cholinergic synapse, we reconstructed a cultured soma–soma chemical synapse model consisting of two identified visceral dorsal 4 (VD4) and left pedal e-1 (LPeD1) neurons from the snail, Lymnaea stagnalis, in vitro, and used it to determine how lidocaine affects cholinergic synaptic transmission. METHODS: The response to acetylcholine and excitatory postsynaptic potential (EPSP) amplitude was recorded in the reconstructed chemical synaptic transmission model composed of VD4 and LPeD1 neurons. The currents for acetylcholine measurements were made under voltage-clamp in the presynaptic VD4 and postsynaptic LPeD1 neurons. RESULTS: Lidocaine inhibited both EPSP and the response for acetylcholine of the postsynaptic neuron. EPSP amplitude was reduced in a voltage-dependent manner in the presynaptic neuron, and lidocaine induced a hyperpolarization shift of the voltage-dependent inactivation curves of EPSP amplitude. CONCLUSIONS: Lidocaine inhibits cholinergic synaptic transmission with a voltage-dependent inactivation of EPSP amplitude through the membrane potential depolarization of presynaptic neurons.
影响因子：
5
作者：
Zhiling Xiong;Zhiling Xiong;Gary R. Strichartz;Gary R. Strichartz
通讯作者：
Zhiling Xiong;Zhiling Xiong;Gary R. Strichartz;Gary R. Strichartz