Origin of bimodal fluorescence enhancement factors of Chlorobaculum tepidum reaction centers on silver island films.
Origin of bimodal fluorescence enhancement factors of Chlorobaculum tepidum reaction centers on silver island films.
复制标题
温热绿杆菌反应双峰荧光增强因子的起源集中在银岛膜上。
DOI：
10.1002/1873-3468.12292
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发表时间：
2016
期刊：
影响因子：
3.5
通讯作者：
Mackowski S
中科院分区：
文献类型：
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作者：
Mackowski S
我们专注于等离子体激元诱导的荧光ofChlorobaculum tepidum反应中心的光谱依赖性。当沉积在银岛膜上时，它们表现出高达60倍的荧光增强。增强因子对激发波长的依赖性与等离子体结构的吸收光谱无关。特别是，Fenna-Matthews-Olson（FMO）蛋白的一个（或多个）三聚体的存在揭示了其自身在589 nm处激发的增强因子的双峰分布，该波长对应于FMO的细菌叶绿素吸收和RC的核心。我们的结论是，多发色团复合物的结构可以在很大程度上影响等离子体激元激发的影响，这是很重要的组装功能的生物杂交系统的背景下。
We focus on the spectral dependence of plasmon‐induced enhancement of fluorescence ofChlorobaculum tepidumreaction centers. When deposited on silver island film, they exhibit up to a 60‐fold increase in fluorescence. The dependence of enhancement factors on the excitation wavelength is not correlated with the absorption spectrum of the plasmonic structure. In particular, the presence of one (or multiple) trimers of the Fenna–Matthews–Olson (FMO) protein reveals itself in bimodal distribution of enhancement factors for the excitation at 589 nm, the wavelength corresponding to bacteriochlorophyll absorption of FMO and the core of the RC. We conclude that the structure of multichromophoric complexes can substantially affect the impact of plasmonic excitations, which is important in the context of assembling functional biohybrid systems.