A retrospective: use of Escherichia coli as a vehicle to study phospholipid synthesis and function.
A retrospective: use of Escherichia coli as a vehicle to study phospholipid synthesis and function.
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DOI：
10.1016/j.bbalip.2012.08.007
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发表时间：
2013-03
期刊：
影响因子：
--
通讯作者：
Dowhan W
中科院分区：
文献类型：
--
作者：
Dowhan W
虽然对个体磷脂及其合成的研究始于20世纪20年代的S，但直到60年代初S，尤金·肯尼迪利用大肠杆菌才开始对细菌磷脂代谢的研究。20世纪60年代末，S利用哺乳动物组织研究中已有的信息，绘制了大肠杆菌中磷脂生物合成的基本蓝图。1970年S和1980年S对大多数磷脂生物合成的酶进行了纯化，并鉴定了许多编码这些酶的基因。到20世纪90年代末，S有了条件突变体和零突变体，以及磷脂生物合成每一步的基因克隆。在获得完整的大肠杆菌基因组序列之前，这些基因中的大多数已经被测序。发展出一种在保持细胞活力的同时可以系统地改变磷脂组成的大肠杆菌菌株。零突变，其中磷脂代谢被人工调节的菌株，以及合成在大肠杆菌中找不到的外来脂类的菌株，至今仍被用来确定单个磷脂的特定作用。这篇综述将追溯导致大肠杆菌发展成为研究磷脂合成和功能的潜在机制的优秀模型系统的研究结果，这些机制广泛适用于其他原核和真核系统。
Although the study of individual phospholipids and their synthesis began in the 1920’s first in plants and then mammals, it was not until the early 1960’s that Eugene Kennedy using Escherichia coli initiated studies of bacterial phospholipid metabolism. With the base of information already available from studies of mammalian tissue, the basic blueprint of phospholipid biosynthesis in E. coli was worked out by the late 1960’s. In 1970’s and 1980’s most of the enzymes responsible for phospholipid biosynthesis were purified and many of the genes encoding these enzymes were identified. By the late 1990’s conditional and null mutants were available along with clones of the genes for every step of phospholipid biosynthesis. Most of these genes had been sequenced before the complete E. coli genome sequence was available. Strains of E. coli were developed in which phospholipid composition could be changed in a systematic manner while maintaining cell viability. Null mutants, strains in which phospholipid metabolism was artificially regulated, and strains synthesizing foreign lipids not found in E. coli have been used to this day to define specific roles for individual phospholipid. This review will trace the findings that have led to the development of E. coli as an excellent model system to study mechanisms underlying the synthesis and function of phospholipids that are widely applicable to other prokaryotic and eukaryotic systems.
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影响因子：
4.8
作者：
BOGDANOV, M;DOWHAN, W
通讯作者：
DOWHAN, W
影响因子：
3.2
作者：
BELL, RM
通讯作者：
BELL, RM
影响因子：
4.8
作者：
Bogdanov, M;Umeda, M;Dowhan, W
通讯作者：
Dowhan, W
DOI：
10.1038/139836a0
发表时间：
1937-01-01
期刊：
Nature London
影响因子：
--
作者：
Arton, C.
通讯作者：
Arton, C.
影响因子：
4.8
作者：
Bogdanov, M;Sun, JZ;Dowhan, W
通讯作者：
Dowhan, W