Vascular Tissue Engineering: Advanced Techniques and Gene Editing in Stem Cells for Graft Generation
Vascular Tissue Engineering: Advanced Techniques and Gene Editing in Stem Cells for Graft Generation
复制标题
血管组织工程：用于移植物生成的干细胞先进技术和基因编辑
DOI：
10.1089/ten.teb.2019.0264
复制
发表时间：
2020-07-27
影响因子：
6.4
通讯作者：
Huang, Chien-Ling
中科院分区：
文献类型：
--
作者：
Chen, Sin-Guang;Ugwu, Felix;Huang, Chien-Ling
心血管疾病的普遍发生和自体组织的缺乏，促使组织工程化血管移植物（TEVG）的发展迫在眉睫。目前在支架生产、转基因细胞和基于纳米技术的监测的使用方面的进展已经大大改善了工程组织移植物的长期通畅性。然而，在组织工程的自体材料和基因和细胞的操作方面存在着大量的挑战。这篇综述概述了TEVG目前的发展，并讨论了支架技术的最新改进和基因编辑工具的效率及其填补干细胞和再生疗法现有空白的能力。还回顾了当前用于制造工程组织的三维打印方法的进展以及用于血管组织的特定生物材料。此外，强调了对血管组织工程具有越来越重要意义的天然和合成聚合物。鉴于其在组织工程中的历史意义，提出了动物模型和基于纳米技术的监测用于工程化移植物的临床前评价。尚未完全实现的组织再生的最终成功取决于培养系统、生物材料构建体和干细胞在合适的人工生理环境中的最佳性能。本综述的主要目标是对基于干细胞的血管组织工程的最新进展以及该领域基因编辑和纳米技术系统的发展提供更广泛的见解。此外，使用自体细胞制备个性化水凝胶为支架制造提供了有前途的方法。这些新方法不仅提高了组织工程血管移植物的疗效，而且还提供了对患者具有长期有益影响的潜在治疗选择。
The common occurrence of cardiovascular diseases and the lack of proper autologous tissues prompt and promote the pressing development of tissue-engineered vascular grafts (TEVGs). Current progress on scaffold production, genetically modified cells, and use of nanotechnology-based monitoring has considerably improved the long-term patency of engineered tissue grafts. However, challenges abound in the autologous materials and manipulation of genes and cells for tissue engineering. This review overviews current development in TEVGs and discusses recent improvements in scaffolding techniques and the efficiency of gene-editing tools and their ability to fill the existing gaps in stem cell and regenerative therapies. Current advances in three-dimensional printing approaches for fabrication of engineered tissues are also reviewed together with specific biomaterials for vascular tissues. In addition, the natural and synthetic polymers that hold increasing significance for vascular tissue engineering are highlighted. Both animal models and nanotechnology-based monitoring are proposed for preclinical evaluation of engineered grafts in view of their historical significance in tissue engineering. The ultimate success of tissue regeneration, which is yet to be fully realized, depends on the optimal performance of culture systems, biomaterial constructs, and stem cells in a suitable artificial physiological environment. Impact statement The main goal of this review is to provide a broadened insight on recent advances in stem cell-based vascular tissue engineering and the development of gene-editing and nanotechnology systems in this field. Moreover, the uses of autologous cells to prepare personalized hydrogels offer promising methods for scaffold fabrication. These new approaches not only improve the efficacy of tissue-engineered vascular grafts, but also offer potential therapeutic options with long-term beneficial effects on patients.