Raman spectroscopic characterization of necrotic cell death
Raman spectroscopic characterization of necrotic cell death
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DOI：
10.1117/1.2978061
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发表时间：
2008-09-01
影响因子：
3.5
通讯作者：
Mourant, Judith R.
中科院分区：
文献类型：
--
作者：
Kunapareddy, Nagapratima;Freyer, James P.;Mourant, Judith R.
拉曼光谱已用于估计由人类恶性黑色素瘤细胞系 (MEL-28) 组成的体外模型系统中坏死引起的生化变化。结合缺氧和缺糖来模拟肿瘤中的坏死细胞死亡。在 24、48 和 72 小时进行非增殖活细胞和死细胞的拉曼光谱测量。通过将细胞光谱与蛋白质、脂质、RNA、DNA 和糖原的基础光谱进行拟合，对活细胞和死细胞的生化成分进行定量估计。在死细胞中观察到脂质和 RNA 的相对量减少，而相对蛋白质含量增加。光谱的比较表明死细胞中蛋白质和核酸存在构象变化。这些结果表明拉曼光谱可用于检测肿瘤中的坏死细胞死亡。 (C) 2008 年光电仪器工程师协会。 [DOI：10.1117/1.2978061]
Raman spectroscopy has been used to estimate the biochemical changes due to necrosis in an in vitro model system comprised of a human malignant melanoma cell line (MEL-28). Combined oxygen and glucose deprivation was used to simulate necrotic cell death in tumors. Raman spectroscopy measurements of nonproliferating live cells and dead cells were made at 24, 48, and 72 hours. Quantitative estimates of the biochemical composition of live and dead cells were made by fitting cell spectra to the basis spectra of protein, lipid, RNA, DNA, and glycogen. A decrease in the relative amount of lipid and RNA, and an increase in the relative protein content, were observed in dead cells. A comparison of the spectra indicated the existence of conformational changes in protein and nucleic acids in dead cells. These results suggest that Raman spectroscopy could be used to detect necrotic cell death in tumors. (C) 2008 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.2978061]