Expression and regulation of periostin/OSF-2 gene in rat uterus and human endometrium
Expression and regulation of periostin/OSF-2 gene in rat uterus and human endometrium
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DOI：
10.1507/endocrj.k07-073
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发表时间：
2008-02-01
影响因子：
2
通讯作者：
Taketani, Yuji
中科院分区：
文献类型：
--
作者：
Hiroi, Hisahiko;Momoeda, Mikio;Taketani, Yuji
骨膜蛋白/OSF 2是α v β 3和α v β 5整联蛋白的配体，并激活Akt/PKB途径。最近报道的periostin/OSF-2基因破坏小鼠表明periostin/OSF-2在植入中起重要作用。定量RT-PCR结果显示，注射17 β-雌二醇（E2）后12 h，大鼠子宫中periostin/OSF-2 mRNA的表达量下降至约10%，而注射孕酮（P）后，子宫中periostin/OSF-2 mRNA的表达量没有变化。RT-PCR结果显示periostin/OSF-2在人子宫内膜、培养的人子宫内膜基质细胞（ESCs）和培养的人子宫内膜上皮细胞中均有表达。在ESCs中，骨膜蛋白/OSF-2 mRNA的表达在E2处理后6小时降低至约50%。Periostin/OSF 2 mRNA在人子宫内膜中的表达量在月经周期的增殖中期和分泌早期显著增加，在增殖晚期、分泌中期和分泌晚期显著减少。经E2和P处理7和11 d的ESCs中periostin/OSF 2 mRNA的表达显著降低。免疫组化结果显示，Periostin/OSF-2在增殖早期、增殖中期和分泌早期的子宫内膜间质细胞中表达较强，在分泌晚期的子宫内膜上皮细胞中表达较强。本研究表明periostin/OSF-2在大鼠子宫和人子宫内膜中的表达受卵巢类固醇激素的调节。
Periostin/OSF2 is a ligand for alpha v beta 3 and alpha v beta 5 integrins and activates the Akt/PKB pathway. Recent reports of periostin/OSF2 gene disrupted mice indicate that periostin/OSF-2 plays an important role in implantation. Quantitative RT-PCR revealed that the expression of periostin/OSF-2 mRNA in rat uteri was reduced to approximately 10% at 12 h after 17 beta-estradiol (E2) injection, but was not changed after progesterone (P) injection. RT-PCR revealed the expression of periostin/OSF-2 in human endometrium, Cultured human endometrial stromal cells (ESCs), and cultured human endometrial epithelial cells. In ESCs, the expression of periostin/OSF-2 mRNA was reduced to approximately 50% at 6 h after E2 treatment. The amount of periostin/OSF2 mRNA in human endometrium significantly increased during mid-proliferative and early secretory phases of menstrual cycle, and decreased during late-proliferative, mid-secretory and late secretory phases. The expression of periostin/OSF2 mRNA significantly decreased in ESCs decidualized by treatment with E2 and P for 7 and 11 days. By immunohistochemistry, the expression of periostin/OSF-2 was strongly detected in endometrial stromal cells during early proliferative, mid-proliferative and early secretory phases, and was strongly detected in endometrial epithelial cells during late secretory phase. This study demonstrated that the expression of periostin/OSF-2 is regulated by ovarian steroid hormones in rat uterus and human endometrium.