Synthesis, Characterization, and Oil Recovery Application of Biosurfactant Produced by Indigenous Pseudomonas aeruginosa WJ-1 Using Waste Vegetable Oils
Synthesis, Characterization, and Oil Recovery Application of Biosurfactant Produced by Indigenous Pseudomonas aeruginosa WJ-1 Using Waste Vegetable Oils
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DOI：
10.1007/s12010-011-9501-y
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发表时间：
2012-03
影响因子：
3
通讯作者：
Wenjie Xia;Zhi-Bin Luo;H. Dong;Li Yu;Q. Cui;Y. Bi
中科院分区：
文献类型：
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作者：
Wenjie Xia;Zhi-Bin Luo;H. Dong;Li Yu;Q. Cui;Y. Bi
从我国北方热带采油区分离培养出一株菌株。分离株 WJ-1 的生化特征和部分测序的 16S rRNA 基因与铜绿假单胞菌的培养代表相同。这种细菌能够产生一种生物表面活性剂。成分分析表明，提取的生物表面活性剂由高比例的脂质（~74%，w/w）和碳水化合物（~20%，w/w）以及少量蛋白质（~6%，w/w）组成。当细胞在含有 6.0% (w/v) 葡萄糖和 0.75% (w/v) 硝酸钠并补充有 0.1% (v/v) 元素溶液的基本盐培养基上在 37 °C 和 180 rpm 下生长 96 小时后，获得 50.2 g/l 的最佳产量。发现生物表面活性剂生产的最佳pH值为6.0-8.0。 WJ-1生物表面活性剂的临界胶束浓度为0.014 g/L，可将表面张力降低至24.5 mN/m，乳化煤油至EI24≈95。从时间过程研究获得的结果表明，表面张力降低和乳化潜力以与细胞生长相同的方式增加。然而，生物表面活性剂的最大产量发生在稳定生长期（90 小时后）。薄层色谱、傅里叶变换红外光谱和质谱分析表明提取的生物表面活性剂属于鼠李糖脂。岩心固定器驱油实验表明，该菌株及其生物表面活性剂的采油效率为23.02%残油。
A bacterial strain was isolated and cultured from the oil excavation areas in tropical zone in northern China. The biochemical characteristics and partial sequenced 16S rRNA gene of isolate, WJ-1, was identical to those of cultured representatives of the speciesPseudomonas aeruginosa. This bacterium was able to produce a type of biosurfactant. Compositional analysis revealed that the extracted biosurfactant was composed of high percentage lipid (∼74%,w/w) and carbohydrate (∼20%,w/w) in addition to a minor fraction of protein (∼6%,w/w). The best production of 50.2 g/l was obtained when the cells were grown on minimal salt medium containing 6.0% (w/v) glucose and 0.75% (w/v) sodium nitrate supplemented with 0.1% (v/v) element solution at 37 °C and 180 rpm after 96 h. The optimum biosurfactant production pH value was found to be 6.0–8.0. The biosurfactant of WJ-1, with the critical micelle concentration of 0.014 g/L, could reduce surface tension to 24.5 mN/m and emulsified kerosene up to EI24≈95. The results obtained from time course study indicated that the surface tension reduction and emulsification potential was increased in the same way to cell growth. However, maximum biosurfactant production occurred and established in the stationary growth phase (after 90 h). Thin layer chromatography, Fourier transform infrared spectrum, and mass spectrum analysis indicate the extracted biosurfactant was affiliated with rhamnolipid. The core holder flooding experiments demonstrated that the oil recovery efficiency of strain and its biosurfactant was 23.02% residual oil.