Experimental approaches for solution X-ray scattering and fiber diffraction.
Experimental approaches for solution X-ray scattering and fiber diffraction.
复制标题
溶液 X 射线散射和纤维衍射的实验方法。
DOI：
10.1016/j.sbi.2008.08.002
复制
发表时间：
2008
影响因子：
6.8
通讯作者：
Irving,TC
中科院分区：
文献类型：
--
作者：
Tsuruta,H;Irving,TC
近年来，非晶态系统的X射线散射和衍射重新引起了人们的兴趣，因为焦点从高分辨率研究获得的结构化学信息转移到更大长度尺度的结构生理学。这些技术允许研究孤立的大分子以及在接近生理条件下作为整体的高度组织化的大分子组装体。先进的同步加速器仪器使时间分辨方法成为可能，为许多这些研究增加了一个关键的维度。本文综述了非晶X射线散射和衍射的实验方法，这些方法可用于阐明重要的科学问题，如蛋白质/核酸折叠和大分子组装中的结构-功能关系。
X-ray scattering and diffraction from non-crystalline systems have gained renewed interest in recent years, as focus shifts from the structural chemistry information gained by high-resolution studies to the context of structural physiology at larger length scales. Such techniques permit the study of isolated macromolecules as well as highly organized macromolecular assemblies as a whole under near-physiological conditions. Time-resolved approaches, made possible by advanced synchrotron instrumentation, add a crucial dimension to many of these investigations. This article reviews experimental approaches in non-crystalline X-ray scattering and diffraction that may be used to illuminate important scientific questions such as protein/nucleic acid folding and structure–function relationships in large macromolecular assemblies.