Ultrastructure of islet microcirculation, pericytes and the islet exocrine interface in the HIP rat model of diabetes.
Ultrastructure of islet microcirculation, pericytes and the islet exocrine interface in the HIP rat model of diabetes.
复制标题
糖尿病 HIP 大鼠模型胰岛微循环、周细胞和胰岛外分泌界面的超微结构。
DOI：
10.3181/0709-rm-251
复制
发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
Sowers JR
中科院分区：
文献类型：
--
作者：
Hayden MR;Karuparthi PR;Habibi J;Lastra G;Patel K;Wasekar C;Manrique CM;Ozerdem U;Stas S;Sowers JR
转基因人胰岛淀粉样多肽（HIP）大鼠2型糖尿病（T2DM）模型与T2DM患者胰岛功能和结构变化相似。透射电镜观察胰岛微循环超微结构的变化。将来自雄性Sprague道利大鼠（2、4、8、14月龄）的胰腺组织用作对照（SDC），并与2、4、8和14月龄HIP大鼠模型进行比较。与SDC模型相比，2月龄HIP模型未显示胰岛或微循环重塑变化。与SDC模型相比，4月龄HIP模型显示出显著的毛细血管周围淀粉样蛋白沉积和周细胞足突减少。与SDC相比，8月龄模型显示出与周细胞和β细胞凋亡相关的广泛的胰岛淀粉样蛋白沉积。14月龄HIP模型显示β细胞和胰岛内毛细血管显著减少，胰岛几乎完全被淀粉样变性替代。与SDC相比，在4至14月龄的HIP模型中观察到胰岛外分泌界面区域的细胞结构增加。与胰岛内毛细血管稀疏相反，在胰岛外分泌界面有明显的血管生成。周细胞似乎与胶原沉积、胰岛内脂肪生成和胰岛外分泌界面的血管生成密切相关。上述关于微循环和周细胞的新发现可以帮助研究人员和临床医生更好地了解T2DM的形态学，并为T2DM的预防和治疗提供新的策略。
The transgenic human islet amyloid polypeptide (HIP) rat model of type 2 diabetes mellitus (T2DM) parallels the functional and structural changes in human islets with T2DM. The transmission electron microscope (TEM) was utilized to observe the ultrastructural changes in islet microcirculation. Pancreatic tissue from male Sprague Dawley rats (2, 4, 8, 14 months) were used as controls (SDC) and compared to the 2-, 4-, 8- and 14-month-old HIP rat models. The 2-month-old HIP model demonstrated no islet or microcirculation remodeling changes when compared to the SDC models. The 4-month-old HIP model demonstrated significant pericapillary amyloid deposition and diminution of pericyte foot processes as compared to the SDC models. The 8-month-old model demonstrated extensive islet amyloid deposition associated with pericyte and β-cell apoptosis when compared with SDC. The 14-month-old HIP model demonstrated a marked reduction of β-cells and intra-islet capillaries with near complete replacement of islets by amyloidoses. Increased cellularity in the region of the islet exocrine interface was noted in the 4- to 14-month-old HIP models as compared to SDC. In contrast to intra-islet capillary rarefaction there was noticeable angiogenesis in the islet exocrine interface. Pericytes seemed to be closely associated with collagenosis, intra-islet adipogenesis and angiogenesis in the islet exocrine interface. The above novel findings regarding the microcirculation and pericytes could assist researchers and clinicians in a better morphological understanding of T2DM and lead to new strategies for prevention and treatment of T2DM.