Isolation and Some Properties of Silver Ion-resistant Iron-oxidizing Bacterium Thiobacillus ferrooxidans
Isolation and Some Properties of Silver Ion-resistant Iron-oxidizing Bacterium Thiobacillus ferrooxidans
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抗银离子氧化铁细菌氧化亚铁硫杆菌的分离及部分性质
DOI：
10.1271/bbb1961.45.2037
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
K. Imai
中科院分区：
文献类型：
--
作者：
T. Sugio;T. Tano;K. Imai
一种抗银离子的T.通过在补充有银的自养培养基中继代培养细胞，获得在5 × 10-4 M银存在下生长的氧化亚铁酶。在pH 5 ~ 7范围内，抗性细胞释放的银离子量大于敏感细胞。细胞内的银离子几乎全部分布在细胞壁和细胞膜上。由于还原型谷胱甘肽（GSH）的加入降低了银离子对细胞生长和铁氧化酶的抑制作用，并观察到银离子从膜上的GSH依赖性释放，因此推测GSH在细菌的抗性机制中起重要作用。
A silver ion-resistant T. ferrooxidans which grew in the presence of 5 × 10-4 M silver nitrate was obtained by subculturing cells in autotrophic medium supplemented with silver nitrate. The amount of silver ions released from the resistant-cells at pH 5 to pH 7 was larger than that from the sensitrve-cells. Almost all silver ions in the cells were distributed in the cell-wall and cell-membrane. Since inhibition of cell growth and iron-oxidase by silver ions was decreased by the addition of the reduced form of glutathione (GSH) and a GSH dependent release of silver ions from the membrane was observed, it was proposed that GSH plays an important role in the resistance mechanism of the bacterium.