Gene cloning and recombinant expression of a novel fungal immunomodulatory protein from Trametes versicolor
Gene cloning and recombinant expression of a novel fungal immunomodulatory protein from Trametes versicolor
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DOI：
10.1016/j.pep.2012.01.015
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发表时间：
2012-04-01
影响因子：
1.6
通讯作者：
Chen, Guocan
中科院分区：
文献类型：
--
作者：
Li, Feng;Wen, Hua'an;Chen, Guocan
采用同源克隆的方法，从中药用菌杂色曲霉基因组DNA中获得了一个新基因，命名为FIP-TVC。FIP-TVC是真菌免疫调节蛋白家族的成员，全长336bp，编码111个氨基酸，与其他真菌免疫调节蛋白高度同源。此外，还构建了FIP-TVC的表达系统。结果表明，重组蛋白FIP-TVC可在大肠杆菌中表达，表达的蛋白中约有20%是以可溶性形式存在的。重组FIP-TVC具有凝集小鼠和大鼠红细胞的能力。重组蛋白FIP-TVC可选择性增强小鼠脾细胞IL-1α、IL-2、IL-5、IL-6、肿瘤坏死因子-α和淋巴毒素的表达。皇冠版权所有(C)2012由爱思唯尔公司出版。保留所有权利。
Homology-based cloning was used to obtain a new gene (named FIP-tvc) from the genomic DNA of the Chinese traditional medicinal mushroom Trametes versicolor. FIP-tvc is a member of the fungal immunomodulatory protein family, is composed of 336 bp encoding 111 amino acids, and is highly homologous with other fungal immunomodulatory proteins. In addition, an expression system for FIP-tvc was constructed. The results indicated that recombinant protein FIP-tvc could be expressed in Escherichia coli and that about 20% of the expressed protein was in soluble form. Recombinant FIP-tvc was capable of agglutinating mouse and rat red blood cells. Furthermore, recombinant protein FIP-tvc could selectively enhance the expression of interleukin (IL)-1 alpha, IL-2, IL-5, IL-6, tumor necrosis factor-alpha (TNF-alpha), and lympotoxin (LT) in mouse spleen cells. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.