SSD1, which encodes a plant-specific novel protein, controls plant elongation by regulating cell division in rice.
SSD1, which encodes a plant-specific novel protein, controls plant elongation by regulating cell division in rice.
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DOI：
10.2183/pjab.86.265
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Ashikari M
中科院分区：
文献类型：
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作者：
Asano K;Miyao A;Hirochika H;Kitano H;Matsuoka M;Ashikari M
株高是作物改良的重要性状之一。因此，揭示植株伸长机理，根据育种目标控制株高具有重要意义。在本研究中，我们分析了一个新的矮秆突变体ssd1，它的表型不同于典型的GA或BR相关的矮秆表型。SSD1在茎、根、叶和花等各种器官的伸长过程中表现出多效性缺陷。SSd1还显示异常的细胞卷曲和形状，这表明突变体的正常细胞分裂存在缺陷。基于图谱的克隆和互补试验表明，ssd1突变体的矮化表型是由逆转录转座子插入的一个基因引起的，该基因编码植物特有的蛋白，但生化功能未知。BLAST搜索发现，类似SSD1的基因存在于不同的植物物种中，包括单子叶植物和双子叶植物，但不存在于蕨类和苔藓中。我们的结果表明，SSD1通过控制高等植物的细胞分裂来控制植物的伸长。
Plant height is one of the most important traits in crop improvement. Therefore revealing the mechanism of plant elongation and controlling plant height in accordance with breeding object is important. In this study we analyzed a novel dwarf mutant, ssd1, of which phenotype is different from typical GA- or BR-related dwarf phenotype. ssd1 exhibits pleiotropic defects in elongation of various organs such as stems, roots, leaves, and flowers. ssd1 also shows abnormal cell files and shapes, which suggests defects of normal cell division in the mutant. Map-based cloning and complementation test demonstrated that the dwarf phenotype in ssd1 mutant was caused by insertion of retrotransposon in a gene, which encodes plant-specific protein with unknown biochemical function. A BLAST search revealed that SSD1-like genes exist in diverse plant species, including monocots and dicots, but not fern and moss. Our results demonstrate that SSD1 controls plant elongation by controlling cell division in higher plants.