A novel intracellular protein delivery platform based on single-protein nanocapsules
A novel intracellular protein delivery platform based on single-protein nanocapsules
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DOI：
10.1038/nnano.2009.341
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发表时间：
2010-01-01
影响因子：
38.3
通讯作者：
Lu, Yunfeng
中科院分区：
文献类型：
--
作者：
Yan, Ming;Du, Juanjuan;Lu, Yunfeng
一个普通的细胞含有数千种参与正常细胞功能的蛋白质，而大多数疾病都与这些蛋白质中的一种或多种发生故障有关。蛋白质疗法(1)将蛋白质输送到细胞内以取代功能失调的蛋白质，被认为是治疗疾病最直接和最安全的方法。然而，这种方法的有效性受到了限制，因为它的递送效率低，对细胞中消化蛋白质的蛋白酶的稳定性差。在这里，我们展示了一种基于纳米胶囊的新型给药平台，该平台由一个蛋白质核心和一个薄的可渗透的聚合物外壳组成，可以在不同的pH下降解或保持稳定。不可降解的胶囊显示出长期的稳定性，而可降解的胶囊打破了它们的外壳，使核心蛋白质一旦进入细胞就能活跃起来。多种蛋白质可以高效地输送到细胞中，同时保持低毒，这意味着在成像、治疗和化妆品领域有潜在的应用。
An average cell contains thousands of proteins that participate in normal cellular functions, and most diseases are somehow related to the malfunctioning of one or more of these proteins. Protein therapy(1), which delivers proteins into the cell to replace the dysfunctional protein, is considered the most direct and safe approach for treating disease. However, the effectiveness of this method has been limited by its low delivery efficiency and poor stability against proteases in the cell, which digest the protein. Here, we show a novel delivery platform based on nanocapsules consisting of a protein core and a thin permeable polymeric shell that can be engineered to either degrade or remain stable at different pHs. Non-degradable capsules show long-term stability, whereas the degradable ones break down their shells, enabling the core protein to be active once inside the cells. Multiple proteins can be delivered to cells with high efficiency while maintaining low toxicity, suggesting potential applications in imaging, therapy and cosmetics fields.