Immunophenotyping of blood lymphocytes in childhood - Reference values for lymphocyte subpopulations
Immunophenotyping of blood lymphocytes in childhood - Reference values for lymphocyte subpopulations
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DOI：
10.1016/s0022-3476(97)70200-2
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发表时间：
1997-03-01
影响因子：
5.1
通讯作者：
vanDongen, JJM
中科院分区：
文献类型：
--
作者：
ComansBitter, WM;deGroot, R;vanDongen, JJM
目的：血液淋巴细胞的免疫表型分析是诊断血液和免疫疾病的重要工具，由于生命最初几年免疫系统的成熟和扩展，儿童时期淋巴细胞亚群的相对和绝对大小有所不同，因此我们希望获得儿童时期所有相关血液淋巴细胞亚群的相对和绝对大小的参考值。 研究设计：我们采用裂解全血法对儿童淋巴细胞亚群进行分析。 429 份血液样本来自新生儿 (n = 20)、健康儿童 (n = 358) 和成人 (n = 51)。使用以下年龄组：1周至2个月（n = 13）、2至5个月（n = 46）、5至9个月（n = 105）、9至15个月（n = 70）、15至24个月（n = 33）、2至5岁（n = 33）、5至10岁（n = 35）和10至10岁16岁（n = 23）。结果：我们的结果显示，CD19（+）B淋巴细胞的绝对数量在出生后立即增加两倍，直到2岁时保持稳定，随后从2岁到成年逐渐减少6.5倍，CD3（+）T淋巴细胞在出生后立即增加1.5倍，从2岁到成年减少三倍，CD3（+）/CD4（+）T淋巴细胞的绝对大小亚群与总 CD3(+) 群遵循相同的模式，但 CD3(+)/CD8(+) T 淋巴细胞从出生到 2 岁保持稳定，随后逐渐减少至成年水平的三倍。与 B 和 T 淋巴细胞相反，自然杀伤细胞的绝对数量在生命的前 2 个月内减少近三倍，此后保持稳定。我们的研究还表明，淋巴细胞亚群绝对大小的变化并不总是与其相对大小的变化一致，这表明淋巴细胞亚群的相对计数不能反映其实际大小，因此价值有限。结论：基于本研究，我们强烈建议用于诊断血液学和免疫学疾病的血液淋巴细胞免疫表型分析应基于淋巴细胞亚群的绝对大小，而不是基于淋巴细胞亚群的相对大小。我们的数据可用作血液淋巴细胞免疫表型分析的年龄匹配参考值。
Objective: Immunophenotyping of blood lymphocytes is an important tool in the diagnosis of hematologic and immunologic disorders, Because of maturation and expansion of the immune system in the first years of life, the relative and the absolute size of lymphocyte subpopulations vary during childhood, Therefore we wished to obtain reference values for the relative and the absolute size of all relevant blood lymphocyte subpopulations in childhood.Study design: We used the lysed whole blood method for analysis of lymphocyte subpopulations in 429 blood samples from neonates (n = 20), healthy children (n = 358), and adults (n = 51). The following age groups were used: 1 week to 2 months (n = 13), 2 to 5 months (n = 46), 5 to 9 months (n = 105), 9 to 15 months (n = 70), 15 to 24 months (n = 33), 2 to 5 years (n = 33), 5 to 10 years (n = 35), and 10 to 16 years (n = 23).Results: Our results show that the absolute number of CD19(+) B lymphocytes increases twofold immediately after birth, remains stable until 2 years of age, and subsequently gradually decreases 6.5-fold from 2 years to adult age, The CD3(+) T lymphocytes increase 1.5-fold immediately after birth and decrease threefold from 2 years to adult age, The absolute size of the CD3(+)/CD4(+) T-lymphocyte subpopulation follows the same pattern as the total CD3(+) population, but the CD3(+)/CD8(+) T lymphocytes remain stable from birth up to 2 years of age, followed by a gradual threefold decrease toward adult levels, In contrast to B and T lymphocytes, the absolute number of natural killer cells decreases almost threefold in the first 2 months of life and remains stable thereafter. Our study also showed that changes in the absolute size of lymphocyte subpopulations are not always consistent with changes in their relative size, This demonstrates that the relative counts of lymphocyte subsets do not reflect their actual size and are therefore of limited value.Conclusion: On the basis of this study we strongly recommend that immunophenotyping of blood lymphocytes for the diagnosis of hematologic and immunologic disorders be based on the absolute rather than on the relative size of lymphocyte subpopulations. Our data can be used as age-matched reference values for blood lymphocyte immunophenotyping.