Sensitivity of Five Rapid HIV Tests on Oral Fluid or Finger-Stick Whole Blood: A Real-Time Comparison in a Healthcare Setting
Sensitivity of Five Rapid HIV Tests on Oral Fluid or Finger-Stick Whole Blood: A Real-Time Comparison in a Healthcare Setting
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DOI：
10.1371/journal.pone.0011581
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发表时间：
2010-07-19
期刊：
影响因子：
3.7
通讯作者：
Simon, Francois
中科院分区：
文献类型：
--
作者：
Pavie, Juliette;Rachline, Anne;Simon, Francois
背景资料：几个国家的卫生当局最近建议扩大人体免疫机能丧失病毒（艾滋病毒）抗体检测，包括使用快速检测。几个艾滋病毒快速测试，现在在欧洲获得许可，但他们对血液和/或口腔液中的常规healthcare settings的敏感性是未知的。方法和结果：200名成年人与记录的HIV-1（n = 194）或HIV-2感染（n = 6）进行了前瞻性筛选与五个艾滋病毒快速测试使用口腔液（OF）或手指针刺全血（FSB）。OraQuick Advance rapid HIV 1/2（R）首先应用于OF，然后应用于FSB，而其他检测则按以下顺序应用于FSB：Vikia HIV 1/2（R）、Determine HIV 1-2（R）、Determine（R）HIV-1/2 Ag/Ab Combo（R）和HIV-1/HIV-2（R）。对配对血清样本重复进行FSB阴性检测。随机选择20名HIV血清阴性受试者作为对照，并对结果进行盲读。大多数患者有HIV-1亚型B感染（63.3%），大多数患者正在接受抗逆转录病毒治疗（68.5%）。OraQuick OF、OraQuick FSB、Vikia、Determinate、Determinate Ag/Ab Combo和ELISA的灵敏度分别为86.5%、94.5%、98.5%、94.9%、95.8%和99%（p
Background: Health authorities in several countries recently recommended the expansion of human immunodeficiency virus (HIV) antibody testing, including the use of rapid tests. Several HIV rapid tests are now licensed in Europe but their sensitivity on total blood and/or oral fluid in routine healthcare settings is not known.Methods and Findings: 200 adults with documented HIV-1 (n = 194) or HIV-2 infection (n = 6) were prospectively screened with five HIV rapid tests using either oral fluid (OF) or finger-stick whole blood (FSB). The OraQuick Advance rapid HIV1/2(R) was first applied to OF and then to FSB, while the other tests were applied to FSB, in the following order: Vikia HIV 1/2(R), Determine HIV 1-2(R), Determine(R) HIV-1/2 Ag/Ab Combo(R) and INSTI HIV-1/HIV-2(R). Tests negative on FSB were repeated on paired serum samples. Twenty randomly selected HIV-seronegative subjects served as controls, and the results were read blindly. Most patients had HIV-1 subtype B infection (63.3%) and most were on antiretroviral therapy (68.5%). Sensitivity was 86.5%, 94.5%, 98.5%, 94.9%, 95.8% and 99% respectively, with OraQuick OF, OraQuick FSB, Vikia, Determine, Determine Ag/Ab Combo and INSTI (p