Generation of a purely clonal defective interfering influenza virus.
Generation of a purely clonal defective interfering influenza virus.
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纯克隆缺陷型干扰流感病毒的产生。
DOI：
10.1111/1348-0421.12681
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Noda T.
中科院分区：
文献类型：
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作者：
Yamagata Y;Muramoto Y;Miyamoto S;Shindo K;Nakano M;Noda T.
缺陷干扰（DI）流感病毒在干扰感染性病毒复制的基因片段中携带大的缺失;因此，此类病毒具有抗病毒治疗的潜力。然而，由于DI病毒在没有感染性辅助病毒的帮助下不能自主复制，因此尚未产生未被辅助病毒污染的克隆DI病毒原种。为了克服这个问题，我们使用反向遗传学产生具有PB 2 DI基因的克隆DI病毒，使用稳定表达PB 2蛋白的细胞系扩增克隆DI病毒，并证实其在体外干扰感染性病毒复制的能力。因此，我们的方法适用于获得纯克隆的DI病毒，将有助于理解DI病毒的干扰机制，并可用于开发基于DI病毒的抗病毒药物。
Defective interfering (DI) influenza viruses carry a large deletion in a gene segment that interferes with the replication of infectious virus; thus, such viruses have potential for antiviral therapy. However, because DI viruses cannot replicate autonomously without the aid of an infectious helper virus, clonal DI virus stocks that are not contaminated with helper virus have not yet been generated. To overcome this problem, we used reverse genetics to generate a clonal DI virus with a PB2 DI gene, amplified the clonal DI virus using a cell line stably expressing the PB2 protein, and confirmed its ability to interfere with infectious virus replication in vitro. Thus, our approach is suitable for obtaining purely clonal DI viruses, will contribute to the understanding of DI virus interference mechanisms and can be used to develop DI virus‐based antivirals.