Assays to measure nuclear mechanics in interphase cells.
Assays to measure nuclear mechanics in interphase cells.
复制标题
DOI：
10.1002/0471143030.cb2216s56
复制
发表时间：
2012-09
影响因子：
--
通讯作者：
Lammerding, Jan
中科院分区：
文献类型：
--
作者：
Isermann, Philipp;Davidson, Patricia M;Sliz, Josiah D;Lammerding, Jan
细胞核是所有真核细胞的特征性标志。细胞核的物理性质反映了重要的生物学特征，如染色质组织或核膜组成;它们也可以直接影响细胞功能，例如，当细胞通过狭窄的收缩时，僵硬的细胞核可能会成为限制因素。我们提出了两种互补的技术来探测原子核的力学性质。在第一，核硬度相对于周围的细胞骨架推断从诱导核变形应变应用到细胞在弹性基板上。在第二种方法中，根据通过基于灌注的微加工装置的传输时间推导出核的变形性，该装置的收缩小于核的尺寸。这些互补的方法，可应用于测量大量的活的粘附或悬浮细胞的核硬度，可以帮助确定与疾病或发展相关的核力学的重要变化。
The nucleus is the characteristic hallmark of all eukaryotic cells. The physical properties of the nucleus reflect important biological characteristics, such as chromatin organization or nuclear envelope composition; they can also directly affect cellular function, for example, when cells pass through narrow constrictions, where the stiff nucleus may present a limiting factor. We present two complementary techniques to probe the mechanical properties of the nucleus. In the first, nuclear stiffness relative to the surrounding cytoskeleton is inferred from induced nuclear deformations during strain application to cells on an elastic substrate. In the second approach, nuclear deformability is deduced from the transit time through a perfusion-based microfabricated device with constrictions smaller than the size of the nucleus. These complementary methods, which can be applied to measure nuclear stiffness in large numbers of living adherent or suspended cells, can help identify important changes in nuclear mechanics associated with disease or development.