Real-time PCR investigation into the importance of Fusobacterium necrophorum as a cause of acute pharyngitis in general practice
Real-time PCR investigation into the importance of Fusobacterium necrophorum as a cause of acute pharyngitis in general practice
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DOI：
10.1099/jmm.0.45648-0
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发表时间：
2004-10-01
影响因子：
3
通讯作者：
Ludlam, H
中科院分区：
文献类型：
--
作者：
Aliyu, SH;Marriott, RK;Ludlam, H
坏死梭杆菌被认为是一种严重的危及生命的疾病，其特征是菌血症的原因。急性咽喉痛（Lemierre病）后出现转移性脓肿。然而，F necrophorum的重要性作为一个原因，简单的咽喉痛在社会上是未知的。使用定量实时PCR与引物靶向rpoB基因，100例常规咽拭子收集到的患者提出全科医生咽炎进行了分析F坏死梭菌特异性DNA的存在。并与100例健康人咽拭子进行比较。使用血凝素相关蛋白基因特异性PCR检测，10份临床样本对F necrophorum DNA呈阳性，鉴定为F necrophorum funduliforme亚种。所有健康对照均为阴性（双尾P值= 0.0015; Fisher精确检验）。这些结果表明，F.坏死性喉炎可能比以前认识到的在社区中作为单纯性咽喉痛的原因起更重要的作用。
Fusobacterium necrophorum is recognized as the cause of a severe life-threatening illness characterized by bacteraemia. with metastatic abscesses following an acute sore throat (Lemierre's disease). However, the importance of F necrophorum as a cause of simple sore throat in the community is unknown. Using quantitative real-time PCR with primers targeting the rpoB gene, 100 routine throat swabs collected from patients presenting to general practitioners with pharyngitis were analysed for the presence of F necrophorum-specific DNA. The results were compared with those obtained from throat swabs collected from 100 healthy subjects. Ten clinical samples were positive for F necrophorum DNA, identified as F necrophorum subspecies funduliforme, using a haemagglutinin-related protein gene-specific PCR assay. All the healthy controls were negative (two-tailed P value = 0.0015; Fisher exact test). These findings suggest that F. necrophorum may play a more important role as a cause of simple sore throat in the community than has been previously appreciated.