Coexpression of prodynorphin and corticotrophin-releasing hormone in the rat central amygdala: Evidence of two distinct endogenous opioid systems in the lateral division
Coexpression of prodynorphin and corticotrophin-releasing hormone in the rat central amygdala: Evidence of two distinct endogenous opioid systems in the lateral division
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DOI：
10.1002/cne.21464
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发表时间：
2007-10-20
影响因子：
2.5
通讯作者：
Osborne, Peregrine B.
中科院分区：
文献类型：
--
作者：
Marchant, Nathan J.;Densmore, Valerie S.;Osborne, Peregrine B.
杏仁中央核外侧亚核(CEA)由两组表达促肾上腺皮质激素释放激素(CRH)和脑啡肽的氨基丁酸(GABA)神经元组成。糖皮质激素和其他因素对这些神经肽的表达和释放的调节作用被认为对CEA协调的各种躯体、自主和神经内分泌反应具有调节功能。由于另一种阿片肽强啡肽也被报道在外侧CEA的神经元中表达，本研究利用免疫组织化学方法检测了这种kappa-阿片(KOP)受体偏爱配体的神经元表达。杏仁核延长区的前强啡肽神经元主要分布在外侧CEA的内侧、中央和终纹床核的椭圆形。在CEA中约有1/3的前强啡肽神经元共表达CRH，而在BST中未检测到与CRH共表达。外侧CEA内侧部的Calbindin神经元不表达前强啡肽，间接证据表明脑啡肽神经元不表达前强啡肽。在CEA的丘脑外区CRH神经元中共表达前强啡肽，可能为脑强啡肽/Kop受体系统调节应激反应和其他CRH相关功能提供解剖学基础。
The lateral subdivision of the central nucleus of the amygdala (CeA) comprises two groups of gamma-aminobutyric acid (GABA) neurons that express corticotrophin-releasing hormone (CRH) and enkephalin. Regulation of the expression and release of these neuropeptides by glucocorticoids and other factors has been suggested to have a regulatory function on the diverse somatic, autonomic, and neuroendocrine responses that are coordinated by the CeA. Because another opioid peptide, dynorphin, has been reported to be also expressed by neurons in the lateral CeA, this study examined the neuronal expression of this kappa-opioid (KOP) receptor-preferring ligand by using immunohistochemistry for the precursor peptide prodynorphin. Prodynorphin neurons in the extended amygdala were observed mostly in the medial and central regions of the lateral CeA and the oval of the bed nucleus of the stria terminalis (BST). About one-third of the prodynorphin neurons in the CeA coexpressed CRH, whereas no coexpression with CRH was detected in the BST. Prodynorphin was not expressed by calbindin neurons in the medial part of the lateral CeA, and indirect evidence suggested that it was not expressed by enkephalin neurons. Coexpression of prodynorphin in extrahy-pothalamic CRH neurons in the CeA could provide an anatomical basis for regulation of the stress responses and other CRH-related functions by the brain dynorphin/KOP receptor system.