Taraxacum officinale induces cytotoxicity through TNF-α and IL-1α secretion in Hep G2 cells
Taraxacum officinale induces cytotoxicity through TNF-α and IL-1α secretion in Hep G2 cells
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DOI：
10.1016/j.lfs.2003.07.030
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发表时间：
2004-01-16
期刊：
影响因子：
6.1
通讯作者：
Kim, HM
中科院分区：
文献类型：
--
作者：
Koo, HN;Hong, SH;Kim, HM
Yaraxacum officinale（TO）经常被用作女性疾病（如乳腺癌和子宫癌）以及肝脏和胆囊疾病的治疗药物。一些早期的研究表明TO具有抗肿瘤特性，但其机制仍有待阐明。在这项研究中，我们研究了TO对人肝癌细胞系Hep G2的细胞毒性和细胞因子产生的影响。我们的结果表明，与培养基对照相比，TO使细胞活力降低26%，并显著增加肿瘤坏死因子（TNF）-α和白细胞介素（IL）-1 α的产生（TNF-α约为1.6倍，IL-1 α约为2.4倍，P < 0.05）。此外，TO强烈诱导Hep G2细胞的凋亡，如通过流式细胞术测定的。TNF-α的数量增加。IL-1 α参与了TO诱导的细胞凋亡。这些结果表明，TO通过分泌TNF-α和IL-1 α诱导Hep G2细胞的细胞毒性。(C)2003年爱思唯尔公司All rights reserved.
Yaraxacum officinale (TO) has been frequently used as a remedy for women's disease (e.g. breast and uterus cancer) and disorders of the liver and gallbladder. Several earlier studies have indicated that TO exhibits anti-tumor properties, but its mechanism remains to be elucidated. In this study, we investigated the effect of TO on the cytotoxicity and production of cytokines in human hepatoma cell line, Hep G2. Our results show that TO decreased the cell viability by 26%, and significantly increased the tumor necrosis factor (TNF)-alpha and interleukin (IL)-1alpha production compared with media control (about 1.6-fold for TNF-alpha, and 2.4-fold for IL-1alpha, P < 0.05). Also, TO strongly induced apoptosis of Hep G2 cells as determined by flow cytometry. Increased amounts of TNF-alpha. and IL-1 alpha contributed to TO-induced apoptosis. Anti-TNF-alpha and IL-1 alpha antibodies almost abolished it. These results suggest that TO induces cytotoxicity through TNF-alpha and IL-1 alpha secretion in Hep G2 cells. (C) 2003 Elsevier Inc. All rights reserved.