Elucidation of improving effects of a traditional Japanese herbal formula, hochuekkito comprising Atractylodis rhizoma on Tcell-mediated intestinal mucositis in mice
Elucidation of improving effects of a traditional Japanese herbal formula, hochuekkito comprising Atractylodis rhizoma on Tcell-mediated intestinal mucositis in mice
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阐明含有苍术的日本传统草药配方 Hochuekkito 对 T 细胞介导的小鼠肠道粘膜炎的改善作用
DOI：
10.1002/tkm2.1332
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Hiroshi Odaguchi and Hiroaki Kiyohara
中科院分区：
文献类型：
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作者：
Mari Endo;Shiori Utagawa;Takayuki Nagai;Toshihiko Hanawa;Hiroshi Odaguchi and Hiroaki Kiyohara
aimhochuekkitto已被用于胃肠道免疫抑制。其10种中药成分中，苍术（AtR）和苍术（ALR）以及赤芪（HR）和黄芪（AR）可根据患者症状互换。然而，这些改善胃肠道免疫抑制的配方之间的差异尚不清楚。本研究的目的是比较AtR‐和ALR‐hochuekkkiton在T细胞介导的全身和小肠粘膜炎症模型中的作用。我们还分析了AtR - hochuekkiton在Fas/FasL介导的肠上皮细胞信号级联中抗凋亡的活性成分和作用机制。方法分析茯苓多糖、亲脂性成分和低聚糖对抗CD3抗体诱导的全身和小肠黏膜炎症的影响。分析AtR配方对凋亡信号级联相关分子mRNA表达的影响。结果即使HR和AR互换，satr - hochuekkita也能改善肠黏膜炎症。同时，只有ALR方能抑制全身性炎症，而AtR方即使用AR代替HR也不能改善全身性炎症。AtR方增加了抗CD3小鼠小肠中抗凋亡调节适配器分子的基因mRNA表达。AtR配方中的亲脂性成分改善了小肠黏膜炎症的作用；槲皮素是一种有效成分。中等大小的菊寡糖抑制了小肠中细胞毒性CD8淋巴细胞的募集。结论atr - hochuekkitis适合治疗T淋巴细胞自发活化引起的小肠黏膜炎症。
AimHochuekkitohas been used for gastrointestinal immune depression. Among its 10 herbal components,Atractylodis rhizoma(AtR) andAtractylodis lanceae rhizoma(ALR), and alsoHedysari radix(HR) andAstragali radix(AR), can be interchanged based on the patient's symptoms. However, the difference between these formulas for improving gastrointestinal immune depression is not clear. The aim of this study was to compare the effect of AtR‐ and ALR‐hochuekkkitoon a T‐cell‐mediated systemic and small intestinal mucosal inflammation model. We also analyzed the active ingredients and mechanism of action of AtR‐hochuekkitoon the antiapoptotic effect in the Fas/FasL‐mediated signaling cascade on intestinal epithelial cells.MethodsThe effects ofhochuekkito, lipophilic ingredients, and oligosaccharides on the systemic and small intestinal mucosal inflammation induced by anti‐CD3 antibody were analyzed. The effect of the AtR formula on mRNA expression of molecules related to the apoptotic signaling cascade was analyzed.ResultsAtR‐hochuekkitoimproved intestinal mucosal inflammation even if HR and AR were interchanged. Meanwhile, only the ALR formula suppressed systemic inflammation, whereas the AtR formula failed to improve it even if HR was replaced with AR. AtR formula administration increased gene mRNA expression of antiapoptotic regulatory adapter molecules in the small intestine of anti‐CD3 administered mice. The lipophilic ingredients in the AtR formula improved the effect on the small intestinal mucosal inflammation; liquiritin was an active ingredient. The intermediate‐size inulo‐oligosaccharides suppressed the recruitment of cytotoxic CD8 lymphocytes in the small intestine.ConclusionAtR‐hochuekkitois suitable for the treatment of small intestinal mucosal inflammation induced by spontaneous activation of T lymphocytes.