Biomimetically enhanced demineralized bone matrix for bone regenerative applications.
Biomimetically enhanced demineralized bone matrix for bone regenerative applications.
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DOI：
10.3389/fphys.2015.00292
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发表时间：
2015
影响因子：
4
通讯作者：
Narayanan R
中科院分区：
文献类型：
--
作者：
Ravindran S;Huang CC;Gajendrareddy P;Narayanan R
脱钙骨基质(DBM)是牙科应用最广泛的骨移植材料之一。然而，DBM能否可靠和可预测地诱导骨再生一直是一个令人担忧的问题。DBM的质量取决于几个与施主有关的因素和制造技术。为了标准化质量和实现可靠和可预测的骨再生，我们已经产生了一种生物仿生增强版本的DBM(BE-DBM)，以临床级别的商业DBM为对照。我们通过将细胞来源的成骨细胞外基质(ECM)整合到临床级别的DBM中而产生了BE-DBM。在本研究中，我们对BE-DBM进行了表征，并评估了其诱导人骨髓基质细胞(HMSCs)成骨分化的能力，并与临床级别的商业DBM进行了比较。我们的结果表明，BE-DBM在体外和体内都比DBM含有更多的促成骨因子，并能促进hMSC的分化和矿化基质的形成。根据我们的结果，我们预见BE-DBM有可能取代DBM成为骨移植的首选材料。
Demineralized bone matrix (DBM) is one of the most widely used bone graft materials in dentistry. However, the ability of DBM to reliably and predictably induce bone regeneration has always been a cause for concern. The quality of DBM varies greatly depending on several donor dependent factors and also manufacturing techniques. In order to standardize the quality and to enable reliable and predictable bone regeneration, we have generated a biomimetically-enhanced version of DBM (BE-DBM) using clinical grade commercial DBM as a control. We have generated the BE-DBM by incorporating a cell-derived pro-osteogenic extracellular matrix (ECM) within clinical grade DBM. In the present study, we have characterized the BE-DBM and evaluated its ability to induce osteogenic differentiation of human marrow derived stromal cells (HMSCs) with respect to clinical grade commercial DBM. Our results indicate that the BE-DBM contains significantly more pro-osteogenic factors than DBM and enhances HMSC differentiation and mineralized matrix formation in vitro and in vivo. Based on our results, we envision that the BE-DBM has the potential to replace DBM as the bone graft material of choice.