Cell shape dependent regulation of nuclear morphology.
Cell shape dependent regulation of nuclear morphology.
复制标题
DOI：
10.1016/j.biomaterials.2015.07.017
复制
发表时间：
2015-10
期刊：
影响因子：
14
通讯作者：
Ho CC
中科院分区：
文献类型：
--
作者：
Chen B;Co C;Ho CC
最近的研究表明，肌动蛋白丝对细胞如何控制其核形状至关重要。然而，很少知道膜张力在决定核形态中的相对重要性。在这项研究中，我们使用粘接的微图案衬底来改变细胞的几何形状（宽高比、大小和形状），从而允许直接膜张力或不与细胞核进行膜侧接触，并研究一系列矩形形状的细胞核形状重塑和取向。在这里，我们发现在低细胞长径比下，细胞核的方向受肌动蛋白丝的调节，而高长径比的细胞即使在肌动蛋白聚合被阻断时也能保持细胞核的形状和方向。模型腺癌细胞在细胞核形状的调节方面表现出类似的行为，以响应细胞形状的变化，但肌动蛋白丝在维持细胞形状方面是必不可少的。我们的研究结果强调了通过细胞形状控制调节核形状的两种不同机制，以及成纤维细胞和模型癌细胞在细胞粘附和细胞形状控制方面的差异。
Recent studies suggest that actin filaments are essential in how a cell controls its nuclear shape. However, little is known about the relative importance of membrane tension in determining nuclear morphology. In this study, we used adhesive micropatterned substrates to alter the cellular geometry (aspect ratio, size, and shape) that allowed direct membrane tension or without membrane lateral contact with the nucleus and investigate nuclear shape remodeling and orientation on a series of rectangular shapes. Here we showed that at low cell aspect ratios the orientation of the nucleus was regulated by actin filaments while cells with high aspect ratios can maintain nuclear shape and orientation even when actin polymerization was blocked. A model adenocarcinoma cell showed similar behavior in the regulation of nuclear shape in response to changes in cell shape but actin filaments were essential in maintaining cell shape. Our results highlight the two distinct mechanisms to regulate nuclear shape through cell shape control and the difference between fibroblasts and a model cancerous cell in cell adhesion and cell shape control.