ANOMALOUS BEHAVIOR OF COLLAGEN PEPTIDES ON SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS IS DUE TO THE LOW CONTENT OF HYDROPHOBIC AMINO-ACID-RESIDUES
ANOMALOUS BEHAVIOR OF COLLAGEN PEPTIDES ON SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS IS DUE TO THE LOW CONTENT OF HYDROPHOBIC AMINO-ACID-RESIDUES
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DOI：
10.1093/oxfordjournals.jbchem.a132809
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发表时间：
1980-01-01
影响因子：
2.7
通讯作者：
NAGAI, Y
中科院分区：
文献类型：
--
作者：
HAYASHI, T;NAGAI, Y
材料和方法从小牛皮肤(PEP-SIN处理)制备I型和III型胶原，如别处所述(7)。用0.05M醋酸从蝌蚪背部皮肤中提取胶原蛋白，在酸性和中性pH条件下反复盐析提纯。
MATERIALS AND METHODSType I and Type III collagens from calf skin (pep sin-treated) were prepared as described elsewhere (7). Tadpole collagen was extracted with 0.05 m acetic acid from tadpole back skin and purified by repeated salt precipitation at acidic and neutral pH.