Selective targeting of unipolar brush cell subtypes by cerebellar mossy fibers
Selective targeting of unipolar brush cell subtypes by cerebellar mossy fibers
复制标题
DOI：
10.7554/elife.44964
复制
发表时间：
2019-04-17
期刊：
影响因子：
7.7
通讯作者：
Trussell, Laurence O.
中科院分区：
文献类型：
--
作者：
Balmer, Timothy S.;Trussell, Laurence O.
在前庭小脑，初级传入传递来自单个前庭终器的信号，而来自前庭核的次级传入传递整合的信号。不同苔藓纤维的选择性靶向决定了小脑如何处理前庭信号。我们专注于前庭投射到ON和OFF类的单极刷细胞（UBC），将单个苔藓纤维信号分别转化为持久的兴奋或抑制，并影响颗粒细胞的活动。为了确定这些接触是否确实是选择性的，在小鼠中将连接性从UBC追溯到特定的神经节细胞、毛细胞和前庭器官亚型。我们发现，一个专门的子集的初级传入接触上UBC，但不关UBC，而二级传入接触这两种亚型。在初级和次级传入神经、它们的突触和它们接触的UBC之间观察到了惊人的解剖学差异。因此，每类UBC的功能是通过不同的解剖学途径转化特定的信号。
In vestibular cerebellum, primary afferents carry signals from single vestibular end organs, whereas secondary afferents from vestibular nucleus carry integrated signals. Selective targeting of distinct mossy fibers determines how the cerebellum processes vestibular signals. We focused on vestibular projections to ON and OFF classes of unipolar brush cells (UBCs), which transform single mossy fiber signals into long-lasting excitation or inhibition respectively, and impact the activity of ensembles of granule cells. To determine whether these contacts are indeed selective, connectivity was traced back from UBC to specific ganglion cell, hair cell and vestibular organ subtypes in mice. We show that a specialized subset of primary afferents contacts ON UBCs, but not OFF UBCs, while secondary afferents contact both subtypes. Striking anatomical differences were observed between primary and secondary afferents, their synapses, and the UBCs they contact. Thus, each class of UBC functions to transform specific signals through distinct anatomical pathways.