Duration of estrogen exposure prior to follicle-stimulating hormone stimulation is critical to granulosa cell growth and differentiation in rats.
Duration of estrogen exposure prior to follicle-stimulating hormone stimulation is critical to granulosa cell growth and differentiation in rats.
复制标题
促卵泡激素刺激之前雌激素暴露的持续时间对于大鼠颗粒细胞的生长和分化至关重要。
DOI：
10.1095/biolreprod54.6.1336
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发表时间：
1996
影响因子：
3.6
通讯作者：
Mills,TM
中科院分区：
文献类型：
--
作者：
Reilly,CM;Cannady,WE;Mahesh,VB;Stopper,VS;DeSevilla,LM;Mills,TM
本研究考察了猪黄体细胞是否含有特异性前列腺素（PG） E受体。将PG 600r诱导的青春期前循环后备猪或自然循环后备猪的黄体酶解，通过洗脱获得大、小和混合细胞。初步实验表明，在含1% BSA的结合缓冲液中，pH 7.38, 22°C孵育2 h后，[3hipge2与混合细胞的结合效果最佳。此外，[3HIPGE2]的结合被前列腺素取代，顺序为PGE2 PGE1 > PGF2α»PGI2。通过置换分析[3hipge2]和不同浓度未标记的PGE2，检测混合细胞、大细胞和小细胞的特异性PGE2结合。根据采集卵巢前2天至采集卵巢时母猪血清孕酮浓度升高或降低的情况，将每头母猪分别分为A组（n = 6）和B组（n = 3）。PG 600r诱导的青春期前循环后备母猪的黄体细胞与自然循环后备母猪的PGE受体的亲和力和数量相似（p> 0.1）。A组混合细胞含有一个akd21.1±1.1 nM的PGE结合位点，每个大细胞含有5.0 × 105个位点。无论细胞类型和功能状态如何，PGE受体的亲和力相似（p> 0.1）。大细胞PGE受体浓度高于小细胞（p< 0.05）， A组PGE受体浓度高于B组（p= 0.006）。这些数据表明，PGE受体主要存在于猪大黄体细胞上，PGE受体的浓度似乎与黄体功能状态有关
This study examined whether pig luteal cells contain specific prostaglandin (PG) E receptors. Corpora lutea, from PG 600R-induced cycling prepubertal gilts or naturally cycling gilts, were dissociated enzymatically, and large, small, and mixed cells were obtained by elutriation. Preliminary experiments demonstrated that optimal [3HIPGE2binding to mixed cells was obtained after incubation at 22°C for 2 h in binding buffer containing 1% BSA at pH 7.38. In addition, [3HIPGE2 binding was displaced by prostaglandins in the order of PGE2͌ PGE1 > PGF2α» PGI2. The mixed, large, and small cells were assayed for specific PGE2binding by displacement analysis with [3HIPGE2and varying concentrations of unlabeled PGE2. Each gilt was assigned to group A (n = 6) or group B (n = 3) on the basis of whether the serum progesterone concentrations in that pig had increased or decreased, respectively, from 2 days before ovary collection to the time of ovary collection. Luteal cells from PG 600R-induced cycling prepubertal gilts and naturally cycling gilts had similar (p> 0.1) affinities and numbers of PGE receptors. In group A, the mixed cells contained a PGE binding site with aKdof 21.1 ±1.1 nM and a concentration of 5.0 × 105sites per large cell. Affinities of PGE receptors were similar (p> 0.1) regardless of cell types and functional status. PGE receptor concentrations were higher on large cells than on small cells (p< 0.05) and in group A than in group B (p= 0.006). These data demonstrated that PGE receptors primarily exist on porcine large luteal cells and that the concentration of PGE receptors appeared to be related to corpus luteum functional status