Systemic inflammation following hind-limb ischemia-reperfusion affects brain in neonatal mice.
Systemic inflammation following hind-limb ischemia-reperfusion affects brain in neonatal mice.
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DOI：
10.1159/000164686
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发表时间：
2008
影响因子：
2.9
通讯作者：
Ten VS
中科院分区：
文献类型：
--
作者：
Bianco-Batlles MD;Sosunov A;Polin RA;Ten VS
产前和产后感染和炎症与新生儿的神经损伤有关。然而，没有直接的作用，全身炎症介导的神经损伤已被证明。该研究旨在确定远离大脑的器官缺血再灌注（IR）后的全身炎症是否会导致脑损伤。新生小鼠进行2小时的后肢IR。在48小时的再灌注，大脑中的小胶质细胞和caspase-3的活化进行了检查。评估肺水肿和粒细胞浸润。在再灌注24小时测量循环炎症介质的水平。在另一组小鼠中，测量了大脑和后肢血流的变化。所有数据均与假手术小鼠的数据进行比较。与假手术相比，IR小鼠的肺水肿程度高33%（p = 0.04）。这与显著（p = 0.0006）更大的粒细胞浸润和循环细胞因子水平显著增加相关。与假手术小鼠相比，这些相同小鼠的大脑显示出显著（p = 0.02）更多数量的半胱天冬酶-3免疫阳性细胞和小胶质细胞活化。这些数据表明，在远离脑的器官中IR后的全身炎症可诱导神经炎症和脑促凋亡变化。
Antenatal and postnatal infection and inflammation are associated with neurological injury in neonates. However, no direct role for systemic inflammation in mediating neurodamage has been shown. The study was aimed to determine whether systemic inflammation following ischemia-reperfusion (IR) of an organ remotely located from the brain results in cerebral injury. Neonatal mice were subjected to 2 h of hind-limb IR. At 48 h of reperfusion, brains were examined for activation of microglia and caspase-3. Lungs were assessed for pulmonary edema and granulocyte infiltration. The levels of circulating inflammatory mediators were measured at 24 h of reperfusion. In a separate cohort of mice, changes in the cerebral and hind-limb blood flow were measured. All data were compared to that in sham mice. Compared to shams the degree of pulmonary edema in IR mice was 33% (p = 0.04) greater. This was associated with significantly (p = 0.0006) greater granulocytic infiltration and a markedly increased level of circulating cytokines. The brains of these same mice exhibited significantly (p = 0.02) greater numbers of caspase-3-immunopositive cells and activation of microglia compared to sham mice. These data indicate that systemic inflammation following IR in the organ remote from the brain can induce neuroinflammation and cerebral proapoptotic changes.
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影响因子：
3.6
作者：
Leviton, A;Paneth, N;Pagano, M
通讯作者：
Pagano, M
影响因子：
4.3
作者：
Harkin, DW;Barros, AAB;Campbell, FC
通讯作者：
Campbell, FC
影响因子：
2.7
作者：
Bilbo, SD;Rudy, JW;Maier, SF
通讯作者：
Maier, SF
影响因子：
9.8
作者：
Poggi, SH;Park, J;Spong, CY
通讯作者：
Spong, CY
DOI：
10.1111/j.1749-6632.2002.tb04077.x
发表时间：
2002-01-01
期刊：
NITRIC OXIDE: NOVEL ACTIONS, DELETERIOUS EFFECTS AND CLINICAL POTENTIAL
影响因子：
--
作者：
Liu, B;Gao, HM;Hong, JS
通讯作者：
Hong, JS