Longitudinal effects of hepatitis C virus treatment on hepatic mitochondrial dysfunction assessed by 13C-methionine breath test
Longitudinal effects of hepatitis C virus treatment on hepatic mitochondrial dysfunction assessed by 13C-methionine breath test
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DOI：
10.1111/j.1365-2036.2008.03745.x
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发表时间：
2008-08-15
影响因子：
7.6
通讯作者：
Goetze, O.
中科院分区：
文献类型：
--
作者：
Banasch, M.;Emminghaus, R.;Goetze, O.
背景丙型肝炎病毒（HCV）感染的特点是病毒与肝线粒体相互作用引起显着水平的氧化应激。目的通过非侵入性C-13-蛋氨酸呼气试验（MeBT）检查HCV感染患者的肝线粒体功能，并探讨抗病毒治疗的纵向效应。方法对21名接受pegIFN α和利巴韦林抗病毒治疗的慢性丙型肝炎患者和20名健康对照进行研究。所有具有早期病毒学应答的患者 (n = 15) 在基线、第 12 周、治疗结束和 24 周随访后均进行了 MeBT。 结果 12 名患者实现了持续病毒学应答 (SVR)；三名患者的 HCV-RNA 复制复发。与对照组相比，无论基因型和纤维化阶段如何，HCV 感染者的累积 C-13- 呼出百分比 (cPDR(1.5h)) 均显着降低 (P < 0.001)。抗病毒治疗导致 cPDR(1.5h) 进一步衰减（P < 0.01）。治疗停止后，所有患者的 C-13 呼气量至少恢复到基线值。与基线相比，SVR 甚至与平均 cPDR（1.5 小时）增加 70% 相关。 结论 丙型肝炎病毒感染和抗病毒治疗会协同损害肝线粒体功能，在持续消除病毒后，肝线粒体功能可能会恢复正常。 MeBT 可能是监测肝线粒体功能的有价值的诊断工具，特别是对于患有线粒体合并症的患者。
BackgroundHepatitis C virus (HCV) infection is characterized by remarkable levels of oxidative stress induced by virus interactions with hepatic mitochondria.AimTo examine hepatic mitochondrial function in HCV-infected patients assessed by a non-invasive C-13-methionine breath test (MeBT) and to explore longitudinal effects of antiviral treatment.MethodsTwenty-one patients with chronic hepatitis C undergoing antiviral treatment with pegIFN alpha and ribavirin and 20 healthy controls were studied. MeBT was performed at baseline, week 12, end-of-treatment and after 24 weeks of follow-up in all patients with early virological response (n = 15).ResultsTwelve patients achieved sustained virological response (SVR); three patients relapsed for HCV-RNA replication. Cumulative percentage C-13- exhalation (cPDR(1.5h)) was significantly decreased in HCV-infected individuals compared to controls irrespective of genotype and Fibrosis stage (P < 0.001). Antiviral treatment induced a further decay in cPDR(1.5h) (P < 0.01). After treatment cessation, C-13-exhalation returned at least to baseline values in all patients. SVR was even associated with a mean cPDR(1.5h) increase of 70% compared to baseline.ConclusionsHepatitis C virus infection and antiviral treatment synergistically impair hepatic mitochondrial function, which may return to normal after sustained virus elimination. MeBT may be a valuable diagnostic instrument for monitoring hepatic mitochondrial function in particular in patients with mitochondrial comorbidities.