Enamel inspired nanocomposite fabrication through amelogenin supramolecular assembly
Enamel inspired nanocomposite fabrication through amelogenin supramolecular assembly
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DOI：
10.1016/j.biomaterials.2007.02.016
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发表时间：
2007-07-01
期刊：
影响因子：
14
通讯作者：
Moradian-Oldak, Janet
中科院分区：
文献类型：
--
作者：
Fan, Yuwei;Sun, Zhi;Moradian-Oldak, Janet
通过体外制备方法制造类似牙釉质的结构引起了牙科和材料科学领域的极大兴趣。发育中的牙釉质由磷酸钙矿物质、水和牙釉质基质蛋白（主要是釉原蛋白）组成。为了制备模拟这种组合物的材料，建立了一种通过电解沉积（ELD）同时组装牙釉蛋白和磷酸钙沉淀物的新方法。研究发现，重组全长牙釉蛋白 (rP172) 在 ELD（溶液 pH 值增加后）过程中自组装成纳米链结构，并对在半导体硅片表面生长的磷酸钙纳米晶体平行束的诱导产生显着影响。当使用截短的牙釉蛋白（rP148）时；没有观察到纳米链组装，也没有形成平行束。与加入 rP148 的涂层相比，在 rP172 存在下获得的涂层具有改善的弹性模量和硬度。我们的数据表明，牙釉蛋白-磷灰石复合材料中有序束的形成主要是由牙釉蛋白纳米链组装驱动的，并凸显了这种复合材料未来作为牙科修复材料应用的潜力。 (C) 2007 Elsevier Ltd. 保留所有权利。
Fabricating the structures similar to dental enamel through the in vitro preparation method is of great interest in the fields of dentistry and material sciences. Developing enamel is composed of calcium phosphate mineral, water, and enamel matrix proteins, mainly amelogenins. To prepare a material mimicking such composition a novel approach of simultaneously assembling amelogenin and calcium phosphate precipitates by electrolytic deposition (ELD) was established. It was found that recombinant full-length amelogenin (rP172) self-assembled into nanochain structures during ELD (following increase in solution pH), and had significant effect on the induction of the parallel bundles of calcium phosphate nanocrystals, grown on semiconductive silicon wafer surface. When a truncated amelogenin (rP148) was used; no nanochain assembly was observed, neither parallel bundles were formed. The coating obtained in the presence of rP172 had improved elastic modulus and hardness when compared to the coating incorporated with rP148. Our data suggest that the formation of organized bundles in amelogenin-apatite composites is mainly driven by amelogenin nanochain assembly and highlights the potential of such composite for future application as dental restorative materials. (C) 2007 Elsevier Ltd. All rights reserved.