REPEATED ZOOSPORE EMERGENCE IN SAPROLEGNIA-PARASITICA
REPEATED ZOOSPORE EMERGENCE IN SAPROLEGNIA-PARASITICA
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DOI：
10.1016/s0953-7562(09)81060-5
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发表时间：
1994-07-01
影响因子：
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通讯作者：
SODERHALL, K
中科院分区：
文献类型：
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作者：
DIEGUEZURIBEONDO, J;CERENIUS, L;SODERHALL, K
提出了一种测定寄生水霉菌（Saprolegnia parasitica）离体游动孢子重复出芽期数的方法。使用涡旋包囊游动孢子是可能的，以同步的方式，实现多达六代连续的包囊和横向双鞭毛游动孢子。结果表明，游动孢子游动时间的长短影响游动孢子的产生。在游动孢子成囊后，孢子可以被触发发芽或释放一个新的游动孢子，这取决于外部营养物质的添加。机械诱导包囊后45min内才能触发包囊萌发。因此，现在有可能在该物种中详细研究包囊后的两种可选发育途径：要么反复出现游动孢子，要么形成胚管。
A method of determining the number of phases of repeated zoospore emergence in vitro in the water mould Saprolegnia parasitica was developed. Using vortexing to encyst zoospores it was possible, in a synchronous manner, to achieve up to six consecutive generations of cysts and laterally biflagellated zoospores. It was found that the length of time the zoospores were allowed to swim influenced the number of zoospore generations which could be produced. After zoospore encystment the spores can be triggered to either germinate or release a new zoospore depending upon the addition of external nutrients. Germination of cysts could only be triggered within 45 min of mechanically induced encystment. Thus, it is now possible in this species to study in detail the two alternative developmental pathways which follow after encystment: either repeated zoospore emergence or germ tube formation.