Control of enzymatic browning in potato (Solanum tuberosum L.) by sense and antisense RNA from tomato polyphenol oxidase
Control of enzymatic browning in potato (Solanum tuberosum L.) by sense and antisense RNA from tomato polyphenol oxidase
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DOI：
10.1021/jf001217f
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发表时间：
2001-02-01
影响因子：
6.1
通讯作者：
Tumer, N
中科院分区：
文献类型：
--
作者：
Coetzer, C;Corsini, D;Tumer, N
在花椰菜花叶病毒35S启动子的控制下，番茄多酚氧化酶(PPO)基因表达的正义和反义PPO RNAs抑制了Russet Burbank马铃薯的PPO活性。大田种植了37个不同品系的转基因马铃薯植株。测定了田间采集的块茎中PPO活性和酶促褐变程度。在采集的28个转基因品系的块茎中，有5个品系的块茎表现出减少的褐变。在这些品系中，PPO活性水平与酶促褐变的减少有关。这些结果表明，番茄PPO RNA的正义或反义表达抑制了主要商业马铃薯品种的PPO活性和酶促褐变。番茄PPO RNA在正义方向的表达导致PPO活性和酶促褐变的最大幅度下降，可能是由于共抑制。这些结果表明，在不使用各种食品添加剂的情况下，可以利用紧密相关的异源基因的表达来防止多种粮食作物的酶促褐变。
Polyphenol oxidase (PPO) activity of Russet Burbank potato was inhibited by sense and antisense PPO RNAs expressed from a tomato PPO cDNA under the control of the 35S promoter from the cauliflower mosaic virus. Transgenic Russet Burbank potato plants from 37 different lines were grown in the field. PPO activity and the level of enzymatic browning were measured in the tubers harvested from the field. Of the tubers from 28 transgenic lines that were sampled, tubers from 5 lines exhibited reduced browning. The level of PPO activity correlated with the reduction in enzymatic browning in these lines. These results indicate that expression of tomato PPO RNA in sense or antisense orientation inhibits PPO activity and enzymatic browning in the major commercial potato cultivar. Expression of tomato PPO RNA in sense orientation led to the greatest decrease in PPO activity and enzymatic browning, possibly due to cosuppression. These results suggest that expression of closely related heterologous genes can be used to prevent enzymatic browning in a wide variety of food crops without the application of various food additives.