A new artificial nerve graft containing rolled Schwann cell monolayers
A new artificial nerve graft containing rolled Schwann cell monolayers
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DOI：
10.1002/micr.1016
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发表时间：
2001-01-01
期刊：
影响因子：
2.1
通讯作者：
Cheney, ML
中科院分区：
文献类型：
--
作者：
Hadlock, TA;Sundback, CA;Cheney, ML
本研究假设，通过一种新型的滚动移植物结构，在单层中引入大量的雪旺细胞，可以促进强健的神经再生。目的是将附着的雪旺细胞置于人工神经移植物中，并比较雪旺细胞负载移植物与无细胞移植物和自体移植物的再生情况，从新生Fisher大鼠中分离雪旺细胞。取成年Fisher大鼠的小肠黏膜下层（SIS），切成7 mm × 8 cm的薄片，钉住，将雪旺细胞涂在薄片上，使其达到汇合处，随后卷成层流结构，通过大鼠坐骨神经的7 mm间隙植入（n = 12）。对照动物接受不含雪旺细胞的SIS导管（n = 11）或自体移植物修复（n = 12）。在10.5周时，通过坐骨功能指数和伸肌姿势推力测试测量的雪旺细胞负载移植物的功能再生超过了无细胞移植物，并接近自体移植物的功能再生。这些结果突出了雪旺细胞在神经再生中的作用。在雪旺细胞负载组中，再生结果接近自体移植物水平，表明这种方法最终可能在临床神经修复中有用。(C) 2001 Wiley-Liss, Inc。
This study hypothesized that introducing high numbers of Schwann cells in monolayers via a novel rolled graft architecture would promote robust nerve regeneration. The objective was to place adherent Schwann cells in artificial nerve grafts and to assess regeneration through the Schwann cell-laden grafts compared with that through acellular grafts and autografts, Schwann cells were isolated from neonatal Fisher rats. Small intestinal submucosa (SIS) was harvested from adult Fisher rats, cut into 7 mm x 8 cm pieces, and pinned out, Schwann cells were plated onto the strips, allowed to reach confluence, and subsequently rolled into a laminar structure and implanted across a 7-mm gap in the rat sciatic nerve (n = 12). Control animals received SIS conduits without Schwann cells (n = 11) or autograft repair (n = 12), At 10.5 weeks, functional regeneration through the Schwann cell-laden grafts, measured by both sciatic function index and extensor postural thrust testing, exceeded that through the cell-free grafts and approached that achieved through autografts. These results highlight the role of Schwann cells in nerve regeneration. Regenerative results approaching autograft levels in the Schwann cell-laden group suggest that this methodology may ultimately be useful in clinical nerve repair. (C) 2001 Wiley-Liss, Inc.