Nonuniform gene expression pattern detected along the longitudinal axis in the matured rice leaf.
Nonuniform gene expression pattern detected along the longitudinal axis in the matured rice leaf.
复制标题
成熟水稻叶片中沿纵轴检测到的基因表达模式不均匀
DOI：
10.1038/srep08015
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发表时间：
2015-01-26
影响因子：
4.6
通讯作者：
Zhong S
中科院分区：
文献类型：
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作者：
Li N;Chen YR;Ding Z;Li P;Wu Y;Zhang A;Yu S;Giovannoni JJ;Fei Z;Zhang W;Xiang JZ;Xu C;Liu B;Zhong S
水稻（Oryza sativa）是养活世界一半人口的主要作物，也是重要的单子叶植物模式系统。单子叶植物叶以基瓣方式成熟，并沿纵轴具有明确的发育梯度。然而，人们对其叶片成熟后的转录动态知之甚少。在这项研究中，我们通过将成熟水稻叶片切成七个 3 厘米的片段，重建了成熟水稻叶片的高空间分辨率转录组。我们进行了链特异性 Illumina 测序，以生成每个片段的基因表达谱。我们发现成熟的叶子包含纵向基因表达梯度，其中 6.97% (2,603) 的表达基因沿着七个部分显示出差异表达。叶片转录组表现出从基部积累与初级细胞壁和基本细胞代谢相关的转录物到中部参与光合作用和能量产生的转录物以及向尖端参与分解代谢过程的逐渐过渡。
Rice (Oryza sativa) is a staple crop that supports half the world's population and an important monocot model system. Monocot leaf matures in a basipetal manner, and has a well-defined developmental gradient along the longitudinal axis. However, little is known about its transcriptional dynamics after leaf maturation. In this study, we have reconstructed a high spatial resolution transcriptome for the matured rice leaf by sectioning the leaf into seven 3-cm fragments. We have performed strand-specific Illumina sequencing to generate gene expression profiles for each fragment. We found that the matured leaf contains a longitudinal gene expression gradient, with 6.97% (2,603) of the expressed genes showing differentially expression along the seven sections. The leaf transcriptome showed a gradual transition from accumulating transcripts related to primary cell wall and basic cellular metabolism at the base to those involved in photosynthesis and energy production in the middle, and catabolic metabolism process toward the tip.
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