Isolation and mapping of three rice mutants that showed ectopic expression of KNOX genes in leaves
Isolation and mapping of three rice mutants that showed ectopic expression of KNOX genes in leaves
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DOI：
10.1016/j.plantsci.2009.04.007
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发表时间：
2009-08-01
期刊：
影响因子：
5.2
通讯作者：
Kurata, Nori
中科院分区：
文献类型：
--
作者：
Tsuda, Katsutoshi;Ito, Yukihiro;Kurata, Nori
Knotted1-like homeobox （KNOX）基因对植物茎尖分生组织（shoot apical merisystem， SAM）的形成和维持至关重要，在茎尖分生组织中特异性表达，但在叶片等侧枝器官中被抑制。KNOX基因在侧枝器官中的异位表达导致茎发育异常。为了阐明KNOX基因sam特异性表达的遗传机制，我们在水稻Tos17突变体中鉴定了三个隐性突变体洋葱1 （oni1）、oni2和oni3，它们产生了异常芽。表达分析表明，水稻KNOX基因OSH1、OSH6、OSH15和OSH71在所有突变体叶片中均有异位表达，但OSH43不表达。遗传图谱显示，ONI1位于3号染色体的短臂上，ONI2位于10号染色体的长臂上，ONI3位于9号染色体的长臂上。这些结果表明，水稻叶片中存在多个参与抑制KNOX基因表达的位点。2009爱思唯尔爱尔兰有限公司版权所有。
Knotted1-like homeobox (KNOX) genes are essential for the formation/maintenance of the shoot apical meristem (SAM) in plants, and are specifically expressed in the SAM, but are repressed in lateral organs such as leaves. Ectopic expression of KNOX genes in lateral organs was shown to result in abnormal shoot development. To elucidate genetic mechanisms of the SAM-specific expression of KNOX genes, we identified three recessive mutants onion 1 (oni1), oni2 and oni3 that developed abnormal shoots in a rice Tos17 mutant panel. Expression analysis showed that four rice KNOX genes OSH1, OSH6, OSH15 and OSH71, but not OSH43, were ectopically expressed in all mutant leaves. Genetic mapping revealed that ONI1 was located on a short arm of chromosome 3, ONI2 on a long arm of chromosome 10 and ONI3 on a long arm of chromosome 9. These results suggested the presence of multiple loci involved in the repression KNOX gene expression in rice leaves. (C) 2009 Elsevier Ireland Ltd. All rights reserved.