Physiological and proteomic analyses for seed dormancy and release in the perennial grass of Leymus chinensis
Physiological and proteomic analyses for seed dormancy and release in the perennial grass of Leymus chinensis
复制标题
羊草多年生牧草种子休眠与释放的生理与蛋白质组学分析
DOI：
10.1016/j.envexpbot.2019.02.002
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发表时间：
2019-06-01
影响因子：
5.7
通讯作者：
Mao, Peisheng
中科院分区：
文献类型：
--
作者：
Hou, Longyu;Wang, Mingya;Mao, Peisheng
种子休眠性是决定植物种子萌发和成苗的重要性状。尽管种子休眠的研究广泛，相对较少的研究专门集中在种子休眠的野生多年生植物的机制。羊草是一种营养价值极高的多年生禾本科牧草，是欧亚草原的优势种之一。本研究利用iTRAQ技术分析了野生种质种子成熟后休眠和解除的蛋白质组学特征。基于小麦蛋白质数据库，共鉴定出281个在L.与休眠种子相比，非休眠种子中有188个蛋白质差异降低（变化倍数< 0.8，P < 0.05），93个蛋白质差异增加（变化倍数> 1.2，P < 0.05）。基于COG注释，涉及染色质结构和动力学、细胞内运输、切片和囊泡运输、细胞骨架的蛋白质都有差异增加。相反，参与RNA加工和修饰、细胞壁/膜/包膜生物合成和信号转导机制的蛋白质都有差异地减少。基于Mapman注释和功能富集，减少的蛋白质主要参与光合作用和TCA循环，而增加的蛋白质参与蛋白质靶向、降解和合成、氨基酸代谢、脂质代谢、氧化还原、细胞周期和DNA合成/染色质结构。L.后熟休眠种子是种子从成熟到贮藏的过渡时期。蛋白质组学分析表明，L.结果表明，在低温胁迫下，小白菜种子中微管蛋白、组蛋白和硫氧还蛋白的含量增加，L-抗坏血酸过氧化物酶的含量减少。基于这些结果，我们提出后熟过程中活性氧（ROS）的增加是调节休眠解除的重要驱动因素。通过调节细胞骨架和染色质来调控中国红豆杉种子。这些发现为我们理解多年生牧草休眠控制的机理提供了新的见解。
Seed dormancy is an important trait determining seed germination and seedling establishment of plants. Despite extensive studies on seed dormancy, relatively few studies have specifically focused on mechanisms underlying seed dormancy in wild perennial species. Leymus chinensis is a perennial grass forage with excellent nutritional value and one of the dominant species in the Eurasian steppe. In this study, the proteomic features for seed dormancy and release in the wild germplasm after ripening were analyzed by iTRAQ technology. Based on the wheat (Triticwn aestivum) protein database, a total of 281 differently expressed proteins were identified in L. chinensis non-dormant seeds versus dormant seeds, with 188 differentially decreased (fold change < 0.8, P < 0.05) and 93 differentially increased proteins (fold change > 1.2, P < 0.05). Based on the COG annotation, the proteins involved in chromatin structure and dynamics, intracellular trafficking, section, and vesicular transport, cytoskeleton were all increased differentially. In contrast, the proteins involved in RNA processing and modification, cell wall/membrane/envelope biogenesis, and signal transduction mechanisms were all decreased differentially. Based on the Mapman annotation and function enrichment, the decreased proteins mainly participated in photosynthesis and TCA cycle, while the increased proteins were involved in protein targeting, degradation, and synthesis, amino acid metabolism, lipid metabolism, redox, cell cycle, and DNA synthesis/ chromatin structure. After-ripening of L. chinensis dormant seeds was the transition from maturation to storage. Our proteomic analyses revealed that the dormancy release of L. chinensis seeds was related to the increases in tubulin, histone, and thioredoxins, and the decrease in L- ascorbate peroxidase. Based on these results, we proposed that an increase in reactive oxygen species (ROS) during after-ripening is an important driver to regulate dormancy release in L. chinensis seeds by modulating cytoskeleton and chromatin. These findings provide new insight for our mechanistic understanding of dormancy control in perennial grass forage.