The genetic architecture of response to long-term artificial selection for oil concentration in the maize kernel
The genetic architecture of response to long-term artificial selection for oil concentration in the maize kernel
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DOI：
10.1534/genetics.104.029686
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发表时间：
2004-12-01
期刊：
影响因子：
3.3
通讯作者：
Dudley, JW
中科院分区：
文献类型：
--
作者：
Laurie, CC;Chasalow, SD;Dudley, JW
在生物学中运行时间最长的实验之一中，伊利诺伊大学的研究人员自1896年以来一直选择改变玉米粒的成分。在这里，我们使用所有的关联研究来推断在选择油浓度变化时发生的巨大变化的遗传基础。研究群体是由高选择系和低选择系在第70代杂交产生的，随后是10代的随机交配和500个自交系的衍生。对这些品系进行488个遗传标记的基因分型，并在重复的田间试验中评估油浓度。三种分析方法进行了测试，在模拟检测数量性状基因座（QTL）的能力。最有效的方法是多元回归中的模型选择。该方法共检测到50个QTL，占总遗传变异的50%左右，表明该方法涉及的QTL数量超过50个。QTL效应估计值很小，主要是加性的。约20%的QTL具有负效应（即，不是由亲本差异预测的），这与选择期间的搭便车和小群体大小一致。大量的QTL检测占顺利和持续的选择在整个世纪的反应。
In one of the longest-running experiments in biology, researchers at the University of Illinois have selected for altered composition of the maize kernel since 1896. Here we use all association study to infer the genetic basis of dramatic changes that occurred in response to selection for changes in oil concentration. The study population was produced by a cross between the high- and low-selection lines at generation 70, followed by 10 generations of random mating and the derivation of 500 lines by selfing. These lines were genotyped for 488 genetic markers and the oil concentration was evaluated in replicated field trials. Three methods of analysis were tested in simulations for ability to detect quantitative trait loci (QTL). The most effective method was model selection in multiple regression. This method detected similar to50 QTL accounting for similar to50% of the genetic variance, suggesting that >50 QTL are involved. The QTL effect estimates are small and largely additive. About 20% of the QTL have negative effects (i.e., not predicted by the parental difference), which is consistent with hitchhiking and small population size during selection. The large number of QTL-detected accounts for the smooth and sustained response to-selection throughout the twentieth century.