Assessing Lignin Types To Screen Novel Biomass-Degrading Microbial Strains: Synthetic Lignin as Useful Carbon Source
Assessing Lignin Types To Screen Novel Biomass-Degrading Microbial Strains: Synthetic Lignin as Useful Carbon Source
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DOI：
10.1021/acssuschemeng.5b00961
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发表时间：
2016-02
影响因子：
8.4
通讯作者：
P. Picart;Lotte Wiermans;M. Pérez-Sánchez;Philipp M. Grande;A. Schallmey;P. D. María
中科院分区：
文献类型：
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作者：
P. Picart;Lotte Wiermans;M. Pérez-Sánchez;Philipp M. Grande;A. Schallmey;P. D. María
木质素降解微生物菌株的分离可能导致发现新的生物催化剂-过氧化物酶，漆酶和β-醚酶-可能对木质素增值有用。木质素固有的异质性，加上有时难以获得代表性的木质素，可能成为使用木质素作为碳源进行筛选的障碍。本文比较了有机木质素或合成木质素作为碳源对土壤样品中木质素降解细菌的筛选。在这两种情况下，同样的微生物菌株被分离出来，这表明合成木质素-在实验室规模上直接使用过氧化物酶生产-可以成为微生物筛选的有价值的木质素替代品，并且可以获得足够的数量。同样，用过氧化氢和碳酸二甲酯对有机木质素进行50%和100%的脱芳处理，得到的衍生物也被用作碳源。在这些地方……
The isolation of lignin-degrading microbial strains may lead to the discovery of novel biocatalysts—peroxidases, laccases, and β-etherases—potentially useful for lignin valorization. The inherent heterogeneity of lignin, together with the sometimes difficult accessibility to representative amounts of it, may become a hurdle for using lignin as a carbon source for screening purposes. This communication compares the screening of soil samples for lignin-degrading bacteria using as carbon sources either OrganoCat lignin or synthetically produced lignin. In both cases, the same microbial strains were isolated, suggesting that synthetic lignin—straightforwardly produced using peroxidases at laboratory scale—can be a valuable lignin substitute for microbial screenings and available in sufficient quantities. Likewise, OrganoCat lignin was dearomatized (50% and 100%) with a novel protocol using hydrogen peroxide and dimethylcarbonate, and the obtained derivatives were applied as carbon sources as well. In these ca...