Induction of IGFBP-1 expression by cAMP is associated with histone acetylation status of the promoter region in human endometrial stromal cells.
Induction of IGFBP-1 expression by cAMP is associated with histone acetylation status of the promoter region in human endometrial stromal cells.
复制标题
DOI：
10.1210/en.2012-1420
复制
发表时间：
2012-09
期刊：
影响因子：
4.8
通讯作者：
I. Tamura;H. Asada;R. Maekawa;M. Tanabe;L. Lee;T. Taketani;Y. Yamagata;H. Tamura;N. Sugino
中科院分区：
文献类型：
--
作者：
I. Tamura;H. Asada;R. Maekawa;M. Tanabe;L. Lee;T. Taketani;Y. Yamagata;H. Tamura;N. Sugino
许多基因在人子宫内膜基质细胞（ESC）中受到了决定性化的上调。这项研究调查了表观遗传机制在ESC中通过eSCS的决定而参与IGFBP-1的调节和PRL诱导。子宫内膜与cAMP一起诱导人类皮肤成纤维细胞（HDF）。 ESC中通过染色质免疫沉淀测定的区域高于HDF。 DNA低甲基化水平。 ESC中的PRL启动子和增强子区域。组蛋白的乙酰化水平增加和CCAAT/增强子结合蛋白-β的启动子区域的募集表明，在ESC中，IGFBP-1和PRL的启动子区域的高组蛋白乙酰化状态与诱导有关通过使启动子区域可通过转录因素访问IGFBP-1和PRL基因。
Many genes are up- or down-regulated in human endometrial stromal cells (ESCs) undergoing decidualization. IGF-binding protein-1 (IGFBP-1) and prolactin (PRL) are preferentially expressed during decidualization and are recognized as specific markers of decidualization. This study investigated the involvement of epigenetic mechanisms in the regulation of IGFBP-1 and PRL induction by decidualization in ESCs. ESCs isolated from the proliferative phase endometrium were incubated with cAMP to induce decidualization. Human dermal fibroblasts (HDFs) were used as a nonendometrial control. cAMP induced the expressions of both genes in ESCs but induced the expression of only PRL in HDFs. Histone acetylation levels of the IGFBP-1 promoter region evaluated by chromatin immunoprecipitation assay were higher in ESCs than in HDFs. The IGFBP-1 promoter regions in the two cell types showed similar levels of DNA hypomethylation. The histone acetylation levels and DNA methylation status of the PRL promoter and enhancer regions were similar in the two cell types. cAMP had no significant effects on the histone acetylation levels and DNA methylation status of the IGFBP-1 promoter and the PRL promoter and enhancer regions in ESCs. Cotreatment of HDF with cAMP and histone deacetylase inhibitors induced IGFBP-1 expression, which was accompanied by an increased histone acetylation level and recruitment of CCAAT/enhancer-binding protein-β to the promoter region. These results show that, during decidualization in ESCs, high histone acetylation status of the promoter regions of IGFBP-1 and PRL is associated with the induction of the IGFBP-1 and PRL genes by making the promoter regions accessible to transcriptional factors.