Imaging of single human carcinoma cells in vitro using a clinical whole-body magnetic resonance scanner at 3.0 T
Imaging of single human carcinoma cells in vitro using a clinical whole-body magnetic resonance scanner at 3.0 T
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DOI：
10.1002/mrm.20455
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发表时间：
2005-05-01
影响因子：
3.3
通讯作者：
Bruhn, H
中科院分区：
文献类型：
--
作者：
Pinkernelle, J;Teichgräber, U;Bruhn, H
本研究的目的是检验是否可以在临床3-T扫描仪上仅使用表面线圈通过磁共振(MR)成像来检测标记了氧化铁纳米颗粒的单个人类癌细胞。WiDR人结肠癌细胞负载两种不同包被和大小不同的氧化铁纳米颗粒：氨基硅烷包被(MagForce)和羧基-葡聚糖包被(Resovist)。后者是本文所用的结肠癌细胞系所首选的，并且比包被较小的羧基葡聚糖包被的Resovist(48h)起效快得多(12h)。在光学显微镜控制下，标记的单个癌细胞分布在琼脂糖凝胶中的单分散层中，当使用一个小的圆极化表面线圈和T-2加权的GE序列在3T时，可以检测到点状信号消光。标记的结肠癌细胞的检测阈值为4-5微克铁/10(6)细胞。这项研究消除了对特殊硬件的需要，为临床3-T磁共振扫描仪上的单细胞跟踪开辟了一条新的道路，符合患者研究的要求。《医学评论》53：1187-1192,2005。(C)2005年Wiley-Liss，Inc.
The purpose of the present study was to examine whether single human carcinoma cells labeled with iron oxide nanoparticles could be detected by magnetic resonance (MR) imaging on a clinical 3-T scanner using a surface coil only. WiDr human colon carcinoma cells were loaded with two kinds of iron oxide nanoparticles differing by coating and size: aminosilan-coated (MagForce) and carboxy-dextran-coated particles (Resovist). The latter were preferred by the colon carcinoma cell line used here and taken up much faster (12 h) than the smaller carboxydextran-coated Resovist (48 h). Labeled single carcinoma cells, distributed in an agarose gel in a monodisperse layer as controlled by light microscopy, became detectable as punctuate signal extinctions when using a small circularly polarized surface coil in conjunction with a T-2*-weighted GE sequence at 3 T. The threshold for the detectability, of labeled colon carcinoma cells ranged at a load of 4-5 mu g iron/10(6) cells. Obviating the need for special hardware additions, this study opens a new lane for single-cell tracking on clinical 3-T MR scanners amenable to patient studies. Magn Reson Med 53:1187-1192, 2005. (c) 2005 Wiley-Liss, Inc.