A leaderless mRNA including tRNA-like sequence encodes a small peptide that regulates the expression of GcvB small RNA in Escherichia coli
A leaderless mRNA including tRNA-like sequence encodes a small peptide that regulates the expression of GcvB small RNA in Escherichia coli
复制标题
包含 tRNA 样序列的无前导序列 mRNA 编码一种小肽，可调节大肠杆菌中 GcvB 小 RNA 的表达
DOI：
10.1093/jb/mvac007
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Himeno Hyota
中科院分区：
文献类型：
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作者：
Muto Akira;Goto Simon;Kurita Daisuke;Ushida Chisato;Soma Akiko;Himeno Hyota
在所有的大肠埃希菌基因组中都发现了一个保守的tRNA样序列。该序列与精氨酸-tRNA相似，精氨酸是存在于精氨酸中的。致病性岛和噬菌体。表达实验表明，该序列是编码一个短肽(60个氨基酸：XtpA)的无引导型mRNA的一部分。该基因的缺失突变体比野生型细胞对低浓度的几种氨基糖苷类抗生素更敏感。进一步的分析表明，XtpA正向调节GcvB小RNA的表达，GcvB小RNA参与了对氨基葡萄糖苷类药物的内在抗性。Coli.
A tRNA-like sequence conserved in the genomes of allEscherichia colistrains was found. The sequence resembles arginine-tRNA, which is present inE. colipathogenic islands and phages. Expression experiments revealed that this sequence is a part of a leaderless mRNA encoding a short peptide (60 amino acids: XtpA). A deletion mutant of this gene is more sensitive than wild-type cell to several aminoglycoside antibiotics at low concentrations. Further analyses indicated that XtpA positively regulates the expression of GcvB small RNA, which is involved in the intrinsic resistance to aminoblycosides inE. coli.