Effect of Cordyceps sinensis on the proliferation and differentiation of human leukemic U937 cells
Effect of Cordyceps sinensis on the proliferation and differentiation of human leukemic U937 cells
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DOI：
10.1016/s0024-3205(97)00291-9
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发表时间：
1997-05-16
期刊：
影响因子：
6.1
通讯作者：
Wang, SY
中科院分区：
文献类型：
--
作者：
Chen, YJ;Shiao, MS;Wang, SY
冬虫夏草是一种具有抗肿瘤活性的中草药，可在体内抑制小鼠S180肉瘤的生长。在本研究中，我们分离了冬虫夏草多糖组分(PSCs)，并利用体外培养系统研究了其对人白血病U937细胞增殖和分化的影响。结果表明，PSCs(10mU g/ml)刺激的单个核细胞条件培养液(PSCs-MNC-CM)对U937细胞的增殖有明显的抑制作用，其生长抑制率为78-83%。此外，PSCs-MNC-CM诱导约50%的细胞分化为表达非特异性酯酶(NSE)活性和CD11b、CD14和CD68表面抗原的成熟单核/巨噬细胞。而分化后的U937细胞还具有吞噬和超氧化物生成的功能。然而，单独的PSCs或正常的MNC-CM没有这种作用。正常MNC-CM中干扰素-γ、肿瘤坏死因子-α和白介素1水平很低，而经PSCs刺激后的MNC-CM中它们的水平显著升高。抗体中和实验进一步表明，PSCs-MNC-CM的杀瘤和分化作用主要来源于细胞因子的升高，尤其是干扰素-γ和肿瘤坏死因子-α。这两种细胞因子在抑制U937细胞生长和诱导其分化方面具有协同作用。
Cordyceps sinensis is a herb medicine with antitumor activity capable of suppressing the growth of mouse Sarcoma 180 in vivo. In the present study, we have isolated polysaccharide fraction of Cordyceps sinensis (PSCS) and investigated its effect on the proliferation and differentiation of human leukemic U937 cells using an in vitro culture system. Our results showed that the conditioned medium from PSCS (10 mu g/ml)-stimulated blood mononuclear cells (PSCS-MNC-CM) had an activity that could significantly inhibit the proliferation of U937 cells resulting in a growth inhibition rate of 78-83%. Furthermore, PSCS-MNC-CM treatment induced about 50% of the cells differentiating into mature monocytes/macrophages expressing nonspecific esterase (NSE) activity and the surface antigens of CD11b, CD14, and CD 68. Yet, the differentiated U937 cells also had functions of phagocytosis and superoxide production. However, PSCS alone or normal MNC-CM had no such effects. The levels of interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, and interleukin (IL)-1 were very low in normal MNC-CM, and they were greatly increased in MNC-CM prepared with PSCS stimulation. Antibody neutralization studies further revealed that the tumoricidal and differentiating effects of PSCS-MNC-CM were mainly derived from the elevated cytokines, especially IFN-gamma and TNF-alpha. These two cytokines acted synergistically on inhibiting cell growth and inducing differentiation of the target U937 cells.