Structural and biochemical characterization of inorganic pyrophosphatase from Homo sapiens
Structural and biochemical characterization of inorganic pyrophosphatase from Homo sapiens
复制标题
智人无机焦磷酸酶的结构和生化特征
DOI：
10.1016/j.bbrc.2020.09.139
复制
发表时间：
2020-12-17
影响因子：
3.1
通讯作者：
Li, Lisheng
中科院分区：
文献类型：
--
作者：
Hu, Fen;Huang, Zelong;Li, Lisheng
无机焦磷酸酶（PPase）在能量守恒中起着重要作用，并为许多生物合成途径提供能量。在这里，我们提出了两个三维结构的PPase从智人（Hu-PPase）在2.38埃和3.40埃在不同的结晶条件。两个镁金属离子的Hu-PPase结构复合物中的一个被确定为单体（Hu-PPase-mono），而另一个被确定为二聚体-二聚体（Hu-PPase-dd）。在Hu-PPase-mono的每个不对称单元中，有4个α-螺旋和10个β-链，折叠成桶形结构，并且活性位点含有两个镁离子。像许多物种的PPase一样，我们发现Hu-PPase能够进行自组装。令我们惊讶的是，Hu-PPase自组装的破坏并不影响其酶活性或促进细胞生长的能力。我们的工作揭示了Hu-PPase的不同结构形式，发现Hu-PPase的焦磷酸酶活性与其自组装无关。(C)2020爱思唯尔公司All rights reserved.
Inorganic pyrophosphatase (PPase) plays an essential role in energy conservation and provides energy for many biosynthetic pathways. Here, we present two three-dimensional structures of PPase from Homo sapiens (Hu-PPase) at 2.38 angstrom and 3.40 angstrom in different crystallization conditions. One of the Hu-PPase structures complex of two magnesium metal ions was determined to be a monomer (Hu-PPase-mono) here, while the other one to be a dimer-dimer (Hu-PPase-dd). In each asymmetric unit of Hu-PPase-mono, there are four alpha-helices and ten beta-strands and folds as a barrel structure, and the active site contains two magnesium ions. Like PPases from many species, we found that Hu-PPase was able to undergo self-assembly. To our surprise, disruption of the self-assembly of Hu-PPase did not influence its enzymatic activity or the ability to promote cell growth. Our work uncovered that different structure forms of Hu-PPase and found that the pyrophosphatase activity of Hu-PPase is independent of its self-assembly. (C) 2020 Elsevier Inc. All rights reserved.