Gene expression profiles in rice roots under low phosphorus stress
Gene expression profiles in rice roots under low phosphorus stress
复制标题
DOI：
10.1007/s11103-009-9580-0
复制
发表时间：
2010-03-01
影响因子：
5.1
通讯作者：
Lian, Xingming
中科院分区：
文献类型：
--
作者：
Li, Lihua;Liu, Chao;Lian, Xingming
磷是一种重要的植物常量营养素，是核酸、磷脂、三磷酸腺苷等关键分子的组成部分。磷通常是作物产量潜力的限制养分，因为可被植物直接吸收的可溶性磷浓度很低。植物已经进化出一系列分子和形态上的适应来应对磷的限制。然而，这些对缺磷反应的分子基础还没有完全阐明。本文研究了耐低磷水稻中早18号的基因表达谱。和不耐低磷水稻(Oryza sativa SSP.利用代表水稻基因组所有可能基因的60,000个寡核苷酸(70聚体)的DNA芯片，研究了水稻在低磷胁迫下6小时、24小时和72小时的根，并与对照(正常磷条件)进行了比较。在水稻中早18和拉格鲁的3个时间点中，至少有1,518个和2,358个基因在低磷胁迫下的表达发生了变化。差异表达的基因包括与磷酸盐转运有关的基因、磷酸盐转运以外的转运基因、磷酸酶基因、磷酸酶以外的其他酶基因、初级代谢基因、次生代谢基因等。在低磷处理的早期阶段，中早18根中几个参与糖酵解和TCA循环的基因表达上调，而在拉格鲁根中没有表达。这些结果为进一步研究植物适应低磷的分子机制提供了有用的信息，从而促进了提高作物对磷的利用的研究。
Phosphorus (P), an important plant macronutrient, is a component of key molecules such as nucleic acids, phospholipids and ATP. P is often the limiting nutrient for crop yield potential because of the low concentration of soluble P that can be absorbed directly by plant. Plants have evolved a series of molecular and morphological adaptations to cope with P limitation. However, the molecular bases of these responses to P deficiency have not been thoroughly elucidated. In this report, the gene expression profiles of low-P-tolerant rice Zhongzao 18 (Oryza sativa ssp. Indica) and not-low-P-tolerant rice Lagrue (Oryza sativa ssp. Indica) roots at 6 h, 24 h and 72 h under low P stress were investigated and compared with a control (normal P conditions) profile, using a DNA chip of 60,000 oligos (70 mer) that represented all putative genes of the rice genome. A total of 1,518 and 2,358 genes exhibited alterations in expression in response to low P stress in at least one of the three time points in rice Zhongzao 18 and rice Lagrue, respectively. The differentially expressed genes included those involved in phosphate (Pi) transportation, transportations except for Pi transportation, phosphatase, enzymes other than phosphatase, primary metabolism, secondary metabolism and so on. Several genes involved in glycolysis and TCA cycle were up-regulated during the early stages of low P treatment in rice Zhongzao 18 roots, but not in rice Lagrue roots. The results may provide useful information to further studies of the molecular mechanism of plant adaptation to low P and thus facilitate research in improving P utilization in crop species.